Reference : Intra- and extracellular antioxidant capacities of the new water-soluble form of curc...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/139987
Intra- and extracellular antioxidant capacities of the new water-soluble form of curcumin (NDS27) on stimulated neutrophils and HL-60 cells
English
[en] Capacités antioxydantes intra et extracellulaires d'un nouveau dérivé soluble de la curcumine (NDS27) sur les neutrophiles et HL-60 stimulés.
Derochette, Sandrine mailto [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Franck, Thierry mailto [Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Mouithys-Mickalad, Ange mailto [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Deby-Dupont, Ginette mailto []
Neven, Philippe mailto []
Serteyn, Didier mailto [Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Jan-2013
Chemico-Biological Interactions
Elsevier
201
1-3
49-57
Yes (verified by ORBi)
International
0009-2797
Limerick
Ireland
[en] Curcumin ; ROS ; Neutrophils ; HL-60 cells ; Inflammation ; Myeloperoxidase
[en] Phagocytic cells, especially neutrophils (PMNs) are specialized in the production of reactive oxygen species
(ROS) to kill pathogenic agents, but an excessive ROS production is associated with tissue damages
and inflammatory diseases. Phagocytes are thus prime therapeutic targets to control inflammatory events
associated to ROS production. Nowadays, there is a growing interest for the use of polyphenols to modulate
the inflammatory response. The aim of this work was to study the antioxidant effect of NDS27, a
highly water-soluble form of the polyphenolic molecule curcumin, on in vitro stimulated equine PMNs
and human promyelocytic leukemia cells (HL-60). NDS27 was either pre-incubated with cells and eliminated
before their activation (intracellular effect) or let in the medium (extracellular effect). Our results
indicate that NDS27 significantly and dose-dependently (10 6 M–10 4 M) inhibited the ROS production
in both cell types without affecting their viability. NDS27 was able to cross and interact with cell membrane,
especially for HL-60 cells, while we observed a better intracellular antioxidant effect with PMNs.
The activity of myeloperoxidase (MPO) released by PMNs and HL-60 cells, was decreased by NDS27, but
more efficiently for PMNs. These results suggested that the greater efficiency of NDS27 in PMNs is due to
an inhibitory effect on cells which are more mature for ROS production, probably by targeting the
enzymes implied in respiratory burst like MPO. The modulatory effect of NDS27 on the oxidant activity
of cells involved in immune and inflammatory responses opens perspectives for a therapeutic control of
pathologies with excessive inflammatory reactions.
Centre de l'Oxygène, Recherche et Développement - CORD
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
PURIFICATION DE LA NADPH OXYDASE DES NEUTROPHILES ÉQUINS : MISE AU POINT DE NOUVEAUX OUTILS POUR LA QUANTIFICATION ET LA MESURE SPÉCIFIQUE DE SON ACTIVITÉ ET POUR LA RECHERCHE D'INHIBITEURS DE L'ENZYME
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/139987
10.1016/j.cbi.2012.12.010

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Derochette et al 2013.pdfPublisher postprint500.12 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.