Equine endothelial cell; Anoxia/Reoxygenation; High-Resolution oxygraphy; Gas Chromatography; Electron spine resonance; Reactive Oxygen Species; Free Radicals
Résumé :
[en] The endothelium plays an active role in ischemia/reperfusion injuries. Herein, we report the effect of a single or successive cycles of anoxia/reoxygenation (A/R) on the mitochondrial respiratory function of equine endothelial cells (cultured from carotids) monitored by high resolution oxymetry, and on their production of reactive oxygen species (ROS). ROS were measured by electron paramagnetic resonance (ESR) using POBN and DMPO spin traps, and by gas chromatography (GC) of ethylene released by ROS-induced alpha-keto-gamma-(methylthio)butyric acid (KMB) oxidation. The oxygen consumption significantly decreased with the number of A/R cycles, and POBN-ESR spectra were specific of adducts formed in the cells from superoxide anion. After a one-hour A/R cycle, high intensity DMPO-ESR spectra were observed and assigned to superoxide anion trapping; the GC results confirmed an important production of ROS compared to normoxic cells. These results show that A/R induces mitochondrial alterations in endothelial cells, and strongly stimulates their oxidative activity as demonstrated by ESR and GC methods.
Centre/Unité de recherche :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
de la Rebière de Pouyade, Geoffroy ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Salciccia, Alexandra ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Ceusters, Justine ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Deby-Dupont, Ginette
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Langue du document :
Anglais
Titre :
Production of free radicals and oxygen consumption by primary equine endothelial cells during anoxia-reoxygenation.
Date de publication/diffusion :
2011
Titre du périodique :
Open Biochemistry Journal
eISSN :
1874-091X
Maison d'édition :
Bentham Science Publishers, Pays-Bas
Volume/Tome :
5
Pagination :
52-9
Peer reviewed :
Peer reviewed
Organisme subsidiant :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
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