Reference : Development and characterization of microsatellite loci in Pericopsis elata (Fabaceae...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/136735
Development and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-efficient approach
English
Micheneau, Claire []
Dauby, Gilles []
Bourland, Nils mailto [Université de Liège - ULiège > Forêts, Nature et Paysage > Laboratoire de Foresterie des régions trop. et subtropicales >]
Doucet, Jean-Louis [Université de Liège - ULiège > Forêts, Nature et Paysage > Laboratoire de Foresterie des régions trop. et subtropicales >]
Hardy, Olivier []
2011
American Journal of Botany
Botanical Society of America
e268-e270
Yes (verified by ORBi)
International
0002-9122
1537-2197
St Louis
MO
[en] Fabaceae ; Microsatellites ; Next-generation sequencing ; Pericopsis elata ; Tropical timber tree ; Universal fluorescent-labeled primer
[en] Premise of the study : Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures.
Methods and Results : Microsatellite isolation was performed simultaneously on three distinct species through a newly available procedure that associates multiplex microsatellite enrichment and next-generation sequencing, allowing the rapid and low-cost development of microsatellite-enriched libraries through the use of a 1/32nd GS-FLX plate. Genotyping using M13-like label-
ing in multiplexed reactions allowed additional cost savings. From 72 primers selected for initial screening, 21 positively amplified P. elata , and 11 showed polymorphism with two to 11 alleles per locus and a mean value of 5.4 alleles per locus.
Conclusions : These microsatellite loci will be useful to further investigate the level of genetic variation within and between natural populations of P. elata in Africa.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/136735
10.3732/ajb.1100070

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