Development and characterization of microsatellite loci in Pericopsis elata (Fabaceae) using a cost-efficient approach

Premise of the study : Microsatellite loci were developed in the endangered Pericopsis elata using a combination of low-cost procedures.
Methods and Results : Microsatellite isolation was performed simultaneously on three distinct species through a newly available procedure that associates multiplex microsatellite enrichment and next-generation sequencing, allowing the rapid and low-cost development of microsatellite-enriched libraries through the use of a 1/32nd GS-FLX plate. Genotyping using M13-like label-
ing in multiplexed reactions allowed additional cost savings. From 72 primers selected for initial screening, 21 positively amplified P. elata , and 11 showed polymorphism with two to 11 alleles per locus and a mean value of 5.4 alleles per locus.
Conclusions : These microsatellite loci will be useful to further investigate the level of genetic variation within and between natural populations of P. elata in Africa.