Article (Scientific journals)
Isotopically labeled proteome as an internal standard for multiple reaction monitoring-based biomarker quantification.
Turtoi, Andrei; Castronovo, Vincenzo
2012In Expert Review of Proteomics, 9 (3), p. 245-8
Peer Reviewed verified by ORBi
 

Files


Full Text
(177) TURTOI A EXPERT REVIEW PROTEOMICS 2012.pdf
Publisher postprint (5.38 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
cancer; proteomics; relative protein quantification; serum; target analysis; tissue
Abstract :
[en] Multiple reaction monitoring is a mass spectrometry technology used to selectively identify and quantify a known molecule in a complex mixture. The technology has gained favor in proteomic applications, especially for biomarker quantification in human samples. For this purpose, employment of internal standard consisting of isotopically (heavy) labeled proteins is currently considered the best way of normalizing sample preparation and correcting for different ionization efficiencies. However, synthesis of heavy-labeled proteins is considered laborious and expensive. The work outlined here presents an efficient strategy of utilizing isotope-labeled amino acids in cell culture to produce heavy-labeled proteins. These are then spiked into serum and serve as internal standards to relatively quantify a large number of target proteins. The method has been applied to quantify 72 proteins in the sera of pancreatic cancer patients with remarkable efficiency and accuracy.
Research center :
Giga-Cancer - ULiège
Disciplines :
Oncology
Biochemistry, biophysics & molecular biology
Author, co-author :
Turtoi, Andrei ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Language :
English
Title :
Isotopically labeled proteome as an internal standard for multiple reaction monitoring-based biomarker quantification.
Publication date :
2012
Journal title :
Expert Review of Proteomics
ISSN :
1478-9450
eISSN :
1744-8387
Publisher :
Taylor & Francis, United Kingdom
Volume :
9
Issue :
3
Pages :
245-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 November 2012

Statistics


Number of views
113 (3 by ULiège)
Number of downloads
207 (1 by ULiège)

Scopus citations®
 
2
Scopus citations®
without self-citations
2
OpenCitations
 
1

Bibliography


Similar publications



Contact ORBi