Pyrosequencing of epizootic rabbit enteropathy inocula and rabbit caecal samples.

[en] The aetiological agent of epizootic rabbit enteropathy (ERE) is still unknown although a bacterial infection seems the most likely hypothesis. In this study, amplification of the V5 and V6 regions of 16SrDNA from four virulent and two non-virulent caecal samples was performed using a pyrosequencing platform. The virulent samples did not group in the same cluster. The bacterial flora identified was both different and richer than the cultivable bacterial flora. These findings highlight the need for biomolecular techniques to identify the aetiological agent of ERE.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Huybens, Nathalie

Houeix, J.

Licois, D.

Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes

Marlier, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Médecine des oiseaux, des lagomorphes et des rongeurs

Language :

English

Title :

Pyrosequencing of epizootic rabbit enteropathy inocula and rabbit caecal samples.

Publication date :

2013

Journal title :

Veterinary Journal

ISSN :

1090-0233

eISSN :

1532-2971

Publisher :

Elsevier, London, United Kingdom

Volume :

196

Pages :

109-110

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 19 November 2012

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Bibliography

Bovera F., Nizza S., Marono S., Mallardo K., Piccolo G., Tudisco R., De Martino L., Nizza A. Effect of mannan oligosaccharides on rabbit performance, digestibility and rectal bacterial anaerobic populations during an episode of epizootic rabbit enteropathy. World Rabbit Science 2010, 18:9-16.
Dethlefsen L., Huse S., Sogin M.L., Relman D.A. The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biology 2008, 6:e280.
Euzé J.P., 1998. List of prokaryotic names with standing in nomenclature. In: City, vol. 2010.
Huybens N., Houeix J., Licois D., Mainil J., Marlier D. Inoculation and bacterial analyses of fractions obtained from the reference inoculum TEC4 which experimentally reproduces the epizootic rabbit enteropathy. World Rabbit Science 2009, 17:185-193.
Huybens N., Houeix J., Licois D., Mainil J., Marlier D. Epizootic rabbit enteropathy: Comparison of PCR-based RAPD fingerprints from virulent and non-virulent samples. The Veterinary Journal 2011, 190:416-417.
Ley R.E., Hamady M., Lozupone C., Turnbaugh P.J., Ramey R.R., Bircher J.S., Schlegel M.L., Tucker T.A., Schrenzel M.D., Knight R., Gordon J.I. Evolution of mammals and their gut microbes. Science 2008, 320:1647-1651.
Licois D., Coudert P., Marlier D. Epizootic rabbit enteropathy. Recent Advances in Rabbit Sciences 2006, 163-170. ILVO, Melle, Belgium. L. Maertens, P. Coudert (Eds.).
Licois D., Wyers M., Coudert P. Epizootic rabbit enteropathy: Experimental transmission and clinical characterization. Veterinary Research 2005, 36:601-613.
Margulies M., Egholm M., Altman W.E., Attiya S., Bader J.S., Bemben L.A., Berka J., Braverman M.S., Chen Y.J., Chen Z., Dewell S.B., Du L., Fierro J.M., Gomes X.V., Godwin B.C., He W., Helgesen S., Ho C.H., Irzyk G.P., Jando S.C., Alenquer M.L., Jarvie T.P., Jirage K.B., Kim J.B., Knight J.R., Lanza J.R., Leamon J.H., Lefkowitz S.M., Lei M., Li J., Lohman K.L., Lu H., Makhijani V.B., McDade K.E., McKenna M.P., Myers E.W., Nickerson E., Nobile J.R., Plant R., Puc B.P., Ronan M.T., Roth G.T., Sarkis G.J., Simons J.F., Simpson J.W., Srinivasan M., Tartaro K.R., Tomasz A., Vogt K.A., Volkmer G.A., Wang S.H., Wang Y., Weiner M.P., Yu P., Begley R.F., Rothberg J.M. Genome sequencing in microfabricated high-density picolitre reactors. Nature 2005, 437:376-380.
Marlier D., Dewree R., Lassence C., Licois D., Mainil J., Coudert P., Meulemans L., Ducatelle R., Vindevogel H. Infectious agents associated with epizootic rabbit enteropathy: Isolation and attempts to reproduce the syndrome. The Veterinary Journal 2006, 172:493-500.
Turnbaugh P.J., Hamady M., Yatsunenko T., Cantarel B.L., Duncan A., Ley R.E., Sogin M.L., Jones W.J., Roe B.A., Affourtit J.P., Egholm M., Henrissat B., Heath A.C., Knight R., Gordon J.I. A core gut microbiome in obese and lean twins. Nature 2009, 457:480-484.