[en] MALDI in-source decay (ISD) has been used for the top-down sequencing of proteins.
The use of 1,5-diaminonapthalene (1,5-DAN) gave strong intensity of w ions, which are
informative fragments and can be helpful for the distinction of the isobaric amino acids,
Leu and Ile. Our data suggests that the w fragments are formed from z* radical
fragment by unimolecular dissociation and high abundance of w ions in MALDI-ISD
with 1,5-DAN can be understood as resulting from the low collision rate in the MALDI
plume. The MALDI-ISD with 1,5-DAN could be a useful method for the top-down
sequencing of proteins including discrimination of Leu and Ile near the C-terminal end.
Research Center/Unit :
Giga-Systems Biology and Chemical Biology - ULiège
FP7 - 278346 - VENOMICS - High-throughput peptidomics and transcriptomics of animal venoms for discovery of novel therapeutic peptides and innovative drug development
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] JSPS - Japan Society for the Promotion of Science [JA] CE - Commission Européenne [BE]
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