Discrimination of Isobaric Leu/Ile Residues by MALDI In-source Decay Mass Spectrometry

[en] MALDI in-source decay (ISD) has been used for the top-down sequencing of proteins. The use of 1,5-diaminonapthalene (1,5-DAN) gave strong intensity of w ions, which are informative fragments and can be helpful for the distinction of the isobaric amino acids, Leu and Ile. Our data suggests that the w fragments are formed from z* radical fragment by unimolecular dissociation and high abundance of w ions in MALDI-ISD with 1,5-DAN can be understood as resulting from the low collision rate in the MALDI plume. The MALDI-ISD with 1,5-DAN could be a useful method for the top-down sequencing of proteins including discrimination of Leu and Ile near the C-terminal end.

Research Center/Unit :

Giga-Systems Biology and Chemical Biology - ULiège

Disciplines :

Chemistry
Biochemistry, biophysics & molecular biology

Author, co-author :

Asakawa, Daiki

Smargiasso, Nicolas ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Language :

English

Title :

Discrimination of Isobaric Leu/Ile Residues by MALDI In-source Decay Mass Spectrometry

Publication date :

February 2013

Journal title :

Journal of the American Society for Mass Spectrometry

ISSN :

1044-0305

eISSN :

1879-1123

Publisher :

Spinger, New York, United States

Volume :

Issue :

Pages :

297-300

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://link.springer.com/article/10.1007%2Fs13361-012-0523-7

European Projects :

FP7 - 278346 - VENOMICS - High-throughput peptidomics and transcriptomics of animal venoms for discovery of novel therapeutic peptides and innovative drug development

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique
JSPS - Japan Society for the Promotion of Science
CE - Commission Européenne

Available on ORBi :

since 25 September 2012

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