Cloning and expression analysis of an inducible HSP70 gene from tilapia fish

Amino Acid Sequence; Animals; Base Sequence; Carps/metabolism; *Cloning, Molecular; DNA/chemistry; *Gene Expression; HSP70 Heat-Shock Proteins/chemistry/*genetics; Hot Temperature; Molecular Sequence Data; Promoter Regions, Genetic; RNA, Messenger/metabolism; Regulatory Sequences, Nucleic Acid; Reverse Transcriptase Polymerase Chain Reaction; TATA Box; Tilapia/*genetics; Transfection; Zebrafish/embryology

Abstract :

[en] We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.

Research Center/Unit :

AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Molina, Alfredo

Biemar, Frédéric

Muller, Ferenc; University of Southampton

Iyengar, Arati; University of Southampton

Prunet, Patrick; University of Southampton

Maclean, Norman; University of Southampton

Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Language :

English

Title :

Cloning and expression analysis of an inducible HSP70 gene from tilapia fish

Publication date :

2000

Journal title :

FEBS Letters

ISSN :

0014-5793

eISSN :

1873-3468

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

474

Issue :

Pages :

5-10

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-40B1WS3-2&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=c63d83f731968e344c667a50628c12f5

Available on ORBi :

since 20 May 2009

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