Article (Scientific journals)
Cloning and expression analysis of an inducible HSP70 gene from tilapia fish
Molina, Alfredo; Biemar, Frédéric; Muller, Ferenc et al.
2000In FEBS Letters, 474 (1), p. 5-10
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Keywords :
Amino Acid Sequence; Animals; Base Sequence; Carps/metabolism; *Cloning, Molecular; DNA/chemistry; *Gene Expression; HSP70 Heat-Shock Proteins/chemistry/*genetics; Hot Temperature; Molecular Sequence Data; Promoter Regions, Genetic; RNA, Messenger/metabolism; Regulatory Sequences, Nucleic Acid; Reverse Transcriptase Polymerase Chain Reaction; TATA Box; Tilapia/*genetics; Transfection; Zebrafish/embryology
Abstract :
[en] We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.
Research Center/Unit :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Molina, Alfredo
Biemar, Frédéric
Muller, Ferenc;  University of Southampton
Iyengar, Arati;  University of Southampton
Prunet, Patrick;  University of Southampton
Maclean, Norman;  University of Southampton
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Cloning and expression analysis of an inducible HSP70 gene from tilapia fish
Publication date :
2000
Journal title :
FEBS Letters
ISSN :
0014-5793
eISSN :
1873-3468
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
474
Issue :
1
Pages :
5-10
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 May 2009

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