Article (Scientific journals)
Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene
Molina, Alfredo; Iyengar, Arati; Marins, Luis F. et al.
2001In Biochimica et Biophysica Acta, 1520 (3), p. 195-202
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Keywords :
Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cloning, Molecular; Embryo, Nonmammalian; Genes, Reporter; Microinjections; Molecular Sequence Data; *Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Ribosomal Proteins/chemistry/*genetics; Sequence Alignment; Tilapia/embryology/*genetics; Zebrafish
Abstract :
[en] We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.
Research Center/Unit :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Molina, Alfredo
Iyengar, Arati;  University of Southampton
Marins, Luis F.;  University of Southampton
Biemar, Frédéric
Hanley, Sean;  National University of Ireland
Maclean, Norman;  University of Southampton
Smith, Terry J.;  National University of Ireland
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene
Publication date :
2001
Journal title :
Biochimica et Biophysica Acta
ISSN :
0006-3002
eISSN :
1878-2434
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
1520
Issue :
3
Pages :
195-202
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 May 2009

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