Poly (2-dimethylamino-ethylmethacrylate); red blood cells; polycation; stealth red blood cells; immunocamouflage; cell surface engineering; universal red blood cells
Abstract :
[en] Poly(2-dimethylamino-ethylmethacrylate) (PDMAEMA) is a cationic polymer when dissolved in a 7.4 pH fluid. Owing to its ionic nature, this polycation interacts with the negatively charged cell membrane surface of red blood cells (RBCs). The electrostatic self-assembly of PDMAEMA on RBCs membrane can be employed for inducing the formation of a polymeric shield camouflaging blood group antigens on RBCs as a valuable strategy for developing “universal RBCs” for blood transfusion. The purpose of this research was to evaluate the camouflaging ability of PDMAEMA homopolymers and PDMAEMA-copoly(nethylene glycol) copolymers differing in molecular weight and architecture. Surprisingly, the PDMAEMAs caused a partially masking, no masking, and sensitization of the same RBCs population. The MW and architecture of the polymers as well as temperature of PDMAEMA-RBCs treatment influenced the results observed. Herein, the very particular reactivity of PDMAEMAs and RBCs is analyzed and discussed.
Research Center/Unit :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
COTTIN, Sophie ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Flebus, Luca ; Université de Liège - ULiège > Centre interfacultaire des biomatériaux (CEIB)
Pozos-Guillén, Amaury
Flores, Héctor
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Jolois, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
GERARD, Christiane ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
MAGGIPINTO, Gianni ; Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
Sevrin, Chantal ; Université de Liège - ULiège > Centre interfacultaire des biomatériaux (CEIB)
Grandfils, Christian ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
Poly(2-dimethylamino ethylmethacrylate)-Based Polymers To Camouflage Red Blood Cell Antigens
Publication date :
2012
Journal title :
Biomacromolecules
ISSN :
1525-7797
eISSN :
1526-4602
Publisher :
American Chemical Society, Washington, United States - District of Columbia
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