[en] Skeletal muscle voluntary contractions (VC) and electrical stimulations (ES) were compared in eight healthy men. High-energy phosphates and myoglobin oxygenation were simultaneously monitored in the quadriceps by interleaved H-1- and P-31-NMR spectroscopy. For the VC protocol, subjects performed five or six bouts of 5 min with a workload increment of 10% of maximal voluntary torque (MVT) at each step. The ES protocol consisted of a 13-min exercise with a load corresponding to 10% MVT. For both protocols, exercise consisted of 6-s isometric contractions and 6-s rest cycles. For an identical mechanical level (10% MVT), ES induced larger changes than VC in the P-i-to-phosphocreatine ratio [1.38 +/- 1.14 (ES) vs. 0.13 +/- 0.04 (VC)], pH [6.69 +/- 0.11 (ES) vs. 7.04 +/- 0.07 (VC)] and myoglobin desaturation [43 +/- 15.9 (ES) vs. 6.1 +/- 4.6% (VC)]. ES activated the muscle facing the NMR coil to a greater extent than did VCs when evaluated under identical technical conditions. This metabolic pattern can be interpreted in terms of specific temporal and spatial muscle cell recruitment. Furthermore, at identical levels of energy charge, the muscle was more acidotic and cytoplasm appeared more oxygenated during ES than during VC. These results are in accordance with a preferential recruitment of type II fibers and a relative muscle hyperperfusion during ES.
Adams GR, Harris RT, Woodard D, and Dudley GA. Mapping of electrical muscle stimulation using MRI. J Appl Physiol 74: 532-537, 1993.
Argov Z, Lofberg M, and Arnold DL. Insights into muscle diseases gained by phosphorus magnetic resonance spectroscopy. Muscle Nerve 23: 1316-1334, 2000.
Bertocci LA, Haller RG, Lewis SF, Fleckenstein JL, and Nunnally RL. Abnormal high-energy phosphate metabolism in human muscle phosphofructokinase deficiency. J Appl Physiol 70: 1201-1207, 1991.
Blei ML, Conley KE, and Kushmeriek MJ. Separate measures of ATP utilization and recovery in human skeletal muscle. J Physiol 465: 203-222, 1993.
Brillault-Salvat C, Giacomini E, Wary C, Peynsaert J, Jouvensal L, Bloch G, and Carlier PG. An interleaved heteronuclear NMRI-NMRS approach to non-invasive investigation of exercising human skeletal muscle. Cell Mol Biol (Noisy-legrand) 43: 751-762, 1997.
Carlier PG, Bloch G, Wary C, Giacomini E, Jehenson P, Leroy-Willig A, and Leclerc JHJ. Quantitation of myoglobin using tissue water reference implications for the determination of muscle intracellular oxygen pressure by in vivo 1H NMR spectroscopy. In: Proceedings ISMRM, 1994, p. 1251.
Chance B, Eleff S, Leigh JS Jr., Sokolow D, and Sapega A. Mitochondrial regulation of phosphocreatine/inorganic phosphate ratios in exercising human muscle: a gated 31P NMR study. Proc Natl Acad Sci USA 78: 6714-6718, 1981.
Delitto A, Rose SJ, McKowen JM, Lehman RC, Thomas JA, and Shively RA. Electrical stimulation versus voluntary exercise in strengthening thigh musculature after anterior cruciate ligament surgery. Phys Ther 68: 660-663, 1988.
Dennis SC, Gevers W, and Opie LH. Protons in ischemia: where do they come from; where do they go to? J Mol Cell Cardiol 23: 1077-1086, 1991.
Draper V and Ballard L. Electrical stimulation versus electromyographic biofeedback in the recovery of quadriceps femoris muscle function following anterior cruciate ligament surgery. Phys Ther 71: 455-464, 1991.
Duchateau J, De Montigny L, and Hainaut K. Differential effects of training by electrostimulation and by voluntary contractions. Arch Intern Physiol Biochem 96: P11-P12, 1988.
Eriksson E and Haggmark T. Comparison of isometric muscle training and electrical stimulation supplementing isometric muscle training in the recovery after major knee ligament surgery. A preliminary report. Am J Sports Med 7: 169-171, 1979.
Frank LR, Wong EC, Haseler LJ, and Buxton RB. Dynamic imaging of perfusion in human skeletal muscle during exercise with arterial spin labeling. Magn Reson Med 42: 258-267, 1999.
Giat Y, Mizrahi J, and Levy M. A model of fatigue and recovery in paraplegic's quadriceps muscle subjected to intermittent FES. J Biomech Eng 118: 357-366, 1996.
Gutierrez G and Wulf ME. Lactic acidosis in sepsis: a commentary. Intensive Care Med 22: 6-16, 1996.
Hannerz J. Discharge properties of motor units in relation to recruitment order in voluntary contraction. Acta Physiol Scand 91: 374-385, 1974.
Henneman E and Olson CB. Relations between structure and function in the design of skeletal muscle. J Neurophysiol 28: 581-598, 1965.
Hughson RL, Green HJ, and Sharratt MT. Gas exchange, blood lactate, and plasma catecholamines during incremental exercise in hypoxia and normoxia. J Appl Physiol 79: 1134-1141, 1995.
Iotti S, Lodi R, Frassineti C, Zaniol P, and Barbiroli B. In vivo assessment of mitochondrial functionality in human gastrocnemius muscle by 31P MRS. The role of pH in the evaluation of phosphocreatine and inorganic phosphate recoveries from exercise. NMR Biomed 6: 248-253, 1993.
Kemp GJ and Radda GK. Quantitative interpretation of bioenergetic data from 31P and 1H magnetic resonance spectroscopic studies of skeletal muscle: an analytical review. Magnes Res 10: 43-63, 1994.
Kent-Braun JA, Miller RG, and Weiner MW. Magnetic resonance spectroscopy studies of human muscle. Radiol Clin North Am 32: 313-335, 1994.
Kim CK, Bangsbo J, Strange S, Karpakka J, and Saltin B. Metabolic response and muscle glycogen depletion pattern during prolonged electrically induced dynamic exercise in man. Scand J Rehabil Med 27: 51-58, 1995.
Knaflitz M, Merletti R, and De Luca CJ. Inference of motor unit recruitment order in voluntary and electrically elicited contractions. J Appl Physiol 68: 1657-1667, 1990.
Kramer JF and Mendryk SW. Electrical stimulation as a strenght improvement technique: a review. J Orthop Sports Phys Ther 4: 1657-1667, 1982.
Kukulka CG and Clamann HP. Comparison of the recruitment and discharge properties of motor units in human brachial biceps and adductor pollicis during isometric contractions. Brain Res 219: 45-55, 1981.
Lebon V, Carlier PG, Brillault-Salvat C, and Leroy-Willig A. Simultaneous measurement of perfusion and oxygenation changes using a multiple gradient-echo sequence: application to human muscle study. Magn Reson Imaging 16: 721-729, 1998.
Lexell J, Henriksson-Larsen K, and Sjostrom M. Distribution of different fibre types in human skeletal muscles. 2. A study of cross-sections of whole m vastus lateralis. Acta Physiol Scand 117: 115-122, 1983.
Matheson GO, McKenzie DC, Gheorghiu D, Ellinger DC, Quinney HA, and Allen PS. 31P NMR of electrically stimulated rectus femoris muscle: an in vivo graded exercise model. Magn Reson Med 26: 60-70, 1992.
McCully K, Vanderborne K, Posner JD, and Chance B. Magnetic resonance spectroscopy of muscle bioenergetics. In: NMR in Physiology, edited by Gillis RJ. New York: Academic, 1994, p. 405-412.
Mole PA, Chung Y, Tran TK, Sailasuta N, Hurd R, and Jue T. Myoglobin desaturation with exercise intensity in human gastrocnemius muscle. Am J Physiol Regul Integr Comp Physiol 277: R173-R180, 1999.
Moller P and Sylven C. Myoglobin in human skeletal muscle. Scand J Clin Lab Invest 41: 479-482, 1981.
Park JH, Brown RL, Park CR, McCully K, Cohn M, Haselgrove J, and Chance B. Functional pools of oxidative and glycolytic fibers in human muscle observed by 31P magnetic resonance spectroscopy during exercise. Proc Natl Acad Sci USA 84: 8976-8980, 1987.
Ratkevicius A, Mizuno M, Povilonis E, and Quistorff B. Energy metabolism of the gastrocnemius and soleus muscles during isometric voluntary and electrically induced contractions in man. J Physiol 507: 593-602, 1998.
Ratkevicius A, Skurvydas A, Povilonis E, Quistorff B, and Lexell J. Effects of contraction duration on low-frequency fatigue in voluntary and electrically induced exercise of quadriceps muscle in humans. Eur J Appl Physiol Occup Physiol 77: 462-468, 1998.
Raynaud JS, Carlier PG, Wary C, Duteil S, Leroy-Willig A, Vaughan T, and Nauerth A. Improved methodology for dynamic studies of muscle oxygen metabolism using 1H/31P imaging/spectroscopy protocols. MAGMA 9, Suppl 1: 60, 1999.
Raynaud JS, Duteil S, Vaughan JT, Hennel F, Wary C, Leroy-Willig A, and Carlier PG. Determination of skeletal muscle perfusion using arterial spin labeling NMRI: validation by comparison with venous occlusion plethysmography. Magn Reson Med 46: 305-311, 2001.
Richardson RS. What governs skeletal muscle VO2 max? New evidence. Med Sci Sports Exerc 32: 100-107, 2000.
Richardson RS, Newcomer SC, and Noyszewski EA. Skeletal muscle intracellular PO2 assessed by myoglobin desaturation: response to graded exercise. J Appl Physiol 91: 2679-2685, 2001.
Richardson RS, Noyszewski EA, Kendrick KF, Leigh JS, and Wagner PD. Myoglobin O2 desaturation during exercise. Evidence of limited O2 transport. J Clin Invest 96: 1916-1926, 1995.
Ross BD, Radda GK, Gadian DG, Rocker G, Esiri M, and Falconer-Smith J. Examination of a case of suspected McArdle's syndrome by 31P nuclear magnetic resonance. N Engl J Med 304: 1338-1342, 1981.
Schnall MD, Bolinger L, Renshaw PF, Haselgrove JC, Subramanian VH, Eleff SM, Barlow C, Leigh JS Jr., and Chance B. Multinuclear MR imaging: a technique for combined anatomic and physiologic studies. Radiology 162: 863-866, 1987.
Shoemaker JK and Hughson RL. Adaptation of blood flow during the rest to work transition in humans. Med Sci Sports Exerc 31: 1019-1026, 1999.
Sjoholm H, Sahlin K, Edstrom L, and Hultman E. Quantitative estimation of anaerobic and oxidative energy metabolism and contraction characteristics in intact human skeletal muscle in response to electrical stimulation. Clin Physiol 3: 227-239, 1983.
Spriet LL, Soderlund K, Bergstrom M, and Hultman E. Skeletal muscle glycogenolysis, glycolysis, and pH during electrical stimulation in men. J Appl Physiol 62: 616-621, 1987.
Taylor DJ, Bore PJ, Styles P, Gadian DG, and Radda GK. Bioenergetics of intact human muscle. A 31P nuclear magnetic resonance study. Mol Biol Med 1: 77-94, 1983.
Vandenborne K, McCully E, Kakihira H, Prammer M, Bolinger L, Detre JA, De Meirlier K, Walter G, Chance B, and Leigh JS. Metabolic heterogeneity in human calf muscle during maximal exercise. Proc Natl Acad Sci USA 88: 5714-5718, 1991.
Vanderthommen M, Constant T, and Crielaard JM. Rehabilitation of quadriceps: interest of low frequency electrical stimulation after knee arthroscopy. Kinesither Scient 308: 21-22, 1992.
Vanderthommen M, Depresseux JC, Bauvir P, Degueldre C, Delfiore G, Peters JM, Sluse F, and Crielaard JM. A positron emission tomography study of voluntarily and electrically contracted human quadriceps. Muscle Nerve 20: 505-507, 1997.
Vanderthommen M, Depresseux JC, Dauchat L, Degueldre C, Croisier JL, and Crielaard JM. Spatial distribution of blood flow in electrically stimulated human muscle: a positron emission tomography study. Muscle Nerve 23: 482-489, 2000.
Vanderthommen M, Gilles R, Carlier P, Ciancabilla F, Zahlan O, Sluse F, and Crielaard JM. Human muscle energetics during voluntary and electrically induced isometric contractions as measured by 31P NMR spectroscopy. Int J Sports Med 20: 279-283, 1999.
Wang ZY, Noyszewski EA, and Leigh JS Jr. In vivo MRS measurement of deoxymyoglobin in human forearms. Magn Reson Med 14: 562-567, 1990.
Weiner MW, Moussavi RS, Baker AJ, Boska MD, and Miller RG. Constant relationships between force, phosphate concentration, and pH in muscles with differential fatigability. Neurology 40: 1888-1893, 1990.
Wiener DH, Maris J, Chance B, and Wilson JR. Detection of skeletal muscle hypoperfusion during exercise using phosphorus-31 nuclear magnetic resonance spectroscopy. J Am Coll Cardiol 7: 793-799, 1986.