[en] A new chitinase produced by the moderately halophilic bacterium Virgibacillus marismortui strain M3- 23 was identified and characterized. Distinguishable characteristics of high activity and stability at different pH, temperatures and salinity of M3-23 chitinase are reported. Analysis of the catalytic domain sequence from the enzyme highlighted its relationship to glycosyl hydrolase family 18. Comparison of the deduced chitinase sequence from strain M3-23 to known chitinases from Bacillus species showed low similarity (82%), suggested its novelty. This is the first report
of the characterization of chitinase from the species V. marismortui. The halo- and thermo-tolerant nature of the chitinolytic enzyme allows its potential use in agricultural and industrial applications.
Ahmadian G, Degrassi G, Venturi V, Zeigler DR, Soudi M, Zanguinejad P (2007) Bacillus pumilus SG2 isolated from saline conditions produces and secretes two chitinases. J Appl Microbiol 103:1081-89 (Pubitemid 47493243)
Aunpad R, Panbangred W (2003) Cloning and characterization of the constitutively expressed chitinase c gene from a marine bacterium, Salinivibrio costicola strain 5SM-1. J Biosci Bioeng 96:529-536 (Pubitemid 38091227)
Blaak H, Schnellmann J, Walter S, Henrissat B, Schrempf H (1993) Characteristics of an exochitinase from Streptomyces olivaceoviridis, its corresponding gene, putative protein domains and relationship to other chitinases. Eur J Biochem 214:659-669 (Pubitemid 23188140)
Chang WT, Chen CS, Wang SL (2003) An antifungal chitinase produced by Bacillus cereus with shrimp and crab shell powder as a carbon source. Curr Microbiol 47:102-108
Chiang CL, Chang CT, Sung HY (2003) Purification and properties of chitosanase from a mutant of Bacillus subtilis IMR-NK1. Enzyme Microb Technol 32:260-267
Chuan LD (2006) Review of fungal chitinases. Mycopathology 161:345-60
Ellouze O, Mejri M, Smaali I, Limam F, Marzouki MN (2007) Induction, properties and application of xylmanase activity from Slerotinia Sclerotiorum S2 fungus. J Food Biochem 31:96-107
Essghaier B, Fardeau ML, Cayol JL, Hajlaoui MR, Boudabous A, Jijakli H, Sadfi-Zouaoui N (2009) Biological control of grey mould in strawberry fruits by halophilic bacteria. J Appl Microbiol 106:833-46
Essghaier B, Rouaissi M, Boudabous A, Jijakli H, Sadfi-Zouaoui N (2010) Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26. World J Microbiol Biotechnol 26:977-984
Freeman S, Minzm O, Kolesnik I, Barbul O, Zveibil A, Maymon M, Nitzani Y, Kirshner B, Rav-David D, Bilu A, Dag A, Shafir S, Elad Y (2004) Trichoderma biocontrol of Colletotrichum acutatum and Botrytis cinerea and survival in strawberry. Eur J Plant Pathol 110:361-370 (Pubitemid 38463736)
Gomez Ramirez M, Rojas Avelizapa LI, Rojas Avelizapa NG, Cruz CR (2004) Colloidal chitin stained with Remazol Brillant Blue R® , a useful substrate to select chitinolytic microorganisms and to evaluate chitinases. J Microbiol Method 56:213-219 (Pubitemid 38117180)
Gohel V, Maisuria V, Chhatpar HS (2007) Utilization of various chitinous sources for production of mycolytic enzymes by Pantoea dispersa in bench-top-fermenter. Enzyme Microb Technol 40:1608-1614 (Pubitemid 46482649)
Jung WJ, Kuk JH, Kim KY, Kim TH, Park RD (2005) Purification and characterization of chitinase from Paenibacillus illinoisensis KJA-424. J Microbiol Biotechnol 15:274-280
Leelasuphakul W, Sivanunsakul P, Phongpaichit S (2006) Purification, characterization and synergetic activity of β-1, 3-glucanase and antibiotic extract from an antagonistic Bacillus subtilis NRS 89-24 against rice blast and sheath blight. Enzyme Microb Technol 38:990-997
Liu KB, Li SX (1991) Effect of NaCl on element balance, peroxidase iso-enzyme and protein banding pattern of Lycopersicon leaf cultures and regenerated shoots. Sci Hortic 46:97-107
Margesin R, Schinner S (2001) Potential of halotolerant and moderately halophilic microorganisms for biotechnology. Extremophiles 5:73-83 (Pubitemid 33725466)
Messaï A, Hannachi C, Zid E (2006) In vitro regeneration of NaCladapted tomato plants (Lycopersicon esculentum Mill.). Tropicultura 24(4):221-228
Morimoto K, Karita S, Kimura T, Sakka K, Ohmiya K (1997) Cloning, sequencing, and expression of the gene encoding Clostridium paraputrificum chitinase ChiB and analysis of the functions of novel cadherin-like domains and a chitin-binding domain. J Bacteriol 179:7306-7314 (Pubitemid 27509967)
Niehaus F, Bertoldo C, KahlerM, Antranikian G (1999) Extremophiles as a source of novel enzymes for industrial application. ApplMicrobiol Biotechnol 51:711-729 (Pubitemid 29304743)
Pleban S, Chernin L, Chet I (1997) Chitinolytic activity of endophytic strain of Bacillus cereus. Lett Appl Microbiol 25:284-288 (Pubitemid 27438352)
Rodriguez-Kabana R, Godoy G, Morgan-Jones G, Shelby RA (1983) The determination of soil chitinase activity: conditions for assay and ecological studies. Plant Soil 75:95-106
Roja Avelizapa LI, Cruz CR, Guerro MI, Rodriguez VR, Ibarra JE (1999) Selection and characterization of a proteo-chitinolytic strain of Bacillus thuringiensis, able to grow in shrimp waste media. World J Microbiol Biotechnol 15:299-308
Rothschild LJ, Mancinelli RL (2001) Life in extreme environments. Nature 409:1092-1101 (Pubitemid 32204065)
Sadfi-Zouaoui N, Essghaier B, Hajlaoui MR, Fardeau ML, Cayol JL, Ollivier B, Boudabous A (2008) Ability of moderately halophilic bacteria to control grey mould disease on tomato fruits. J Phytopathol 156:42-52 (Pubitemid 350264796)
Sakai K, Narihara M, Kasama Y, Wakayama M, Moriguchi M (1994) Purification and characterization of thermostable beta-N-acetylhexosaminidase of Bacillus stearothermophilus CH-4 isolated from chitin-containing compost. Appl Environ Microbiol 60:2911-2915 (Pubitemid 24242288)
Sanchez-Porro C, Martin S, Mellado E, Ventosa A (2003) A. Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. J Appl Microbiol 94:295-300 (Pubitemid 36120955)
Schisler DA, Slinnger PJ, Behle RW, Jackson MA (2004) Formulation of Bacillus spp. for biological control of plant diseases. Phytopathology 94:1267-1271 (Pubitemid 39448207)
Sivitil AL, Kirchman DL (1998) A chitin-binding domain in a marine bacterial chitinase and other microbial chitinases: implications for the ecology and evolution of 1,4-β-glycanase. Microbiology 144:1299-1308 (Pubitemid 28233834)
Smaali MI, Gargouri M, Limam F, Fattouch S, Maugard T, Legoy MD, Marzouki N (2003) Production, purification and biochemical characterization of two β-glucosidases from Sclerotinia sclerotiorum. Appl Biochem Biotechnol 11:29-40
Trachuck LA, Revina LP, Shemyakina TM, Chestukhina GG, Stepanov VM (1996) Chitinases of Bacillus licheniformis B6839: isolation and properties. Can J Microbiol 42:307-315 (Pubitemid 26160346)
Tsujibo H, Minoura K, Miyamoto K, Endo H, Moriwaki M, Inamori Y (1993a) Purification and properties of a thermostable chitinase from Streptomyces thermoviolaceus OPC-520. Appl Environ Microbiol 59:620-622 (Pubitemid 23058962)
Tsujibo H, Orikoshi H, Tanno H, Fujimoto K, Miyamoto K, Imada C, Okami Y, Inamori Y (1993b) Cloning, sequence, and expression of a chitinase gene from a marine bacterium, Alteromonas sp. strain O-7. J Bacteriol 175:176-181 (Pubitemid 23016664)
Vaidya RJ, Shah IM, Vyas PR, Chhatpar HS (2001) Production of chitinase and optimization from a novel isolate Alcaligenes xylosoxydans: potential in antifungal biocontrol. World J Microbiol Biotechnol 17:691-696 (Pubitemid 33146394)
Ventosa A, Nieto JJ, Oren A (1998) Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62:504-544 (Pubitemid 28270283)
Wang SL, Lin TY, Yen YH, Liao HF, Chen YJ (2006) Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase. Carbohydr Res 341:2507-2515
Watanabe T, Oyanagi W, Suzuki K, Tanaka H (1990) Chitinase system Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation. J Bacteriol 172:4017-4022 (Pubitemid 20213387)
Wu ML, Chuang YC, Chen JP, Chen CS, Chang MC (2001) Identification and characterization of the three chitin-binding domains within the multidomain chitinase Chi92 from Aeromonas hydrophila JP101. Appl Environ Microbiol 67:5100-5106 (Pubitemid 33701276)