embryo cryopreservation; in vitro maturation; polycystic ovarian syndrome
Abstract :
[en] Purpose: A twin pregnancy was obtained in a patient with polycystic ovary syndrome after the transfer of three in vitro maturation-derived day 3 embryos that has been frozen and thawed. Methods: The patient had received mild hMG stimulation followed by hCG injection. After culture for 24 - 48 h, mature oocytes were fertilized by ICSI. Embryos were cultured until day 3; supernumerary embryos were cryopreserved using a slow protocol. Results: Among 15 nonatretic oocytes, 9 matured, 8 were fertilized. Four embryos were transferred but they did not implant. The subsequent transfer of three frozen - thawed embryos resulted in the delivery of two healthy girls. Conclusions: These results indicate that a pregnancy could be obtained with in vitro maturation-derived day-3 frozen - thawed embryos.
Disciplines :
Genetics & genetic processes Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Godin, P. A.
Gaspard, Olivier ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Thonon, Fabienne ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Jouan, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > Gynécologie-Obstétrique CHR
Wijzen, F.
Dubois, Michel ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Language :
English
Title :
Twin pregnancy obtained with frozen-thawed embryos after in vitro maturation in a patient with polycystic ovarian syndrome
Publication date :
August 2003
Journal title :
Journal of Assisted Reproduction and Genetics
ISSN :
1058-0468
eISSN :
1573-7330
Publisher :
Kluwer Academic/Plenum Publishers, New York, United States - New York
Rizk B, Smitz J: Ovarian hyperstimulation syndrome after superovulation using GnRH agonists for IVF and related products. Hum Reprod 1992;7:320-327
Child TJ, Phillips SJ, Abdul-Jalil AK, Gulekli B, Tan SL: A comparison of in vitro maturation and in vitro fertilization for women with polycystic ovaries. Obstet Gynecol 2002;100:665-670
Yoon HG, Yoon SH, Son WY, Lee SW. Park SP, Im KS, Lim JH: Pregnancies resulting from in vitro matured oocytes collected from women with regular menstrual cycle. J Assist Reprod Genet 2001;18:325-329
Lassalle B, Testart J, Renard JP: Human embryo features that influence the success of cryopreservation with the use of 1,2-propanediol. Fertil Steril 1985;44:645-651
Veeck LL, Wortham JW, Jr, Witmyer J, Sandow BA, Acosta AA, Garcia JE, Jones GS, Jones HW, Jr: Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril 1983;39:594-602
Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK: Pregnancy after in vitro fertilization of human follicular oocytes collected from non-stimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991;55:109-113
Trounson A, Wood C, Kausche A: In vitro maturation and fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62:353-362
Gaspard O, Godin PA, Jouan C, Dubois M, Thonon F, Hincourt N, Foidart JM: In-vitro maturation of oocytes: Towards a daily practice? In 17th Annual Meeting of the European Society of Human Reproduction and Embryology, Lausanne, Switzerland, July 2001, Abstr. 0-105
Liu J, Katz E, Garcia JE, Compton G, Baramki TA: Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. Fertil Steril 1997;67:566-568
Chian RC, Buckett WM, Too LL, Tan SL: Pregnancies resulting from in vitro matured oocytes retrieved from patients with polycystic ovary syndrome after priming with human chorionic gonadotropin. Fertil Steril 1999;72:639-642
Choi TS, Mori M, Kohmoto K, Shoda Y: Beneficial effect of serum on fertilizability of mouse oocytes matured in vitro. J Reprod Fertil 1987;79:565-568
Nagy ZP, Cecile J, Liu J, Loccufier A, Devroey P, Van Steirteghem A: Pregnancy and birth after intracytoplasmic injection of in vitro matured germinal-vesicle stage oocytes: Case report. Fertil Steril 1996;65:1047-1050
Ducibella T: The cortical reaction and development of activation competence in mammalian oocytes. Hum Reprod Update 1996;2:29-42
Ducibella T, Duffy P, Reindollar R, Su B: Changes in the distribution of mouse oocyte cortical granules and ability to undergo cortical reaction during gonadotropin-stimulated meiotic maturation and aging in vivo. Biol Reprod 1990;43:870-876
Bachvarova R: Incorporation of tritiated adenosine into mouse ovum RNA. Dev Biol 1974;40:52-58
Jahn CL, Baran MM, Bachvarova R: Stability of RNA synthesized by the mouse oocyte during its major growth phase. J Exp Zool 1976;197:161-171
Telford NA, Watson A, Schultz G: Transition from maternal to embryonic control in early mammalian development: A comparison of several species. Mol Reprod Dev 1990;26:90-100
Thibault C: Hammond Memorial Lecture. Are follicular maturation and oocyte maturation independent processes? J Reprod Fertil 1977;51:1-15
Thibault C, Gerard M, Menezo Y: Preovulatory and ovulatory mechanisms in oocyte maturation. J Reprod Fertil 1975;45:605-610
Wynn P, Picton HM, Krapez JA, Rutherford AJ, Balen AH, Gosden RG: Pretreatment with follicle stimulating hormone promotes the numbers of human oocytes reaching metaphase II by in-vitro maturation. Hum Reprod 1998;13:3132-3138
Hillier SG, Whitelaw PF. Smyth CD: Follicular oestrogen synthesis: The 'two-cell, two-gonadotrophin' model revisited. Mol Cell Endocrinol 1994;100:51-54
McGee E, Spears N, Mminami S, Hsu SY, Chun SY, Billig H, Hsueh AJ: Preantral ovarian follicles in serum-free culture: Suppression of apoptosis after activation of the cyclic guanosine 3′,5′-monophosphate pathway and stimulation of growth and differentiation by follicle-stimulating hormone. Endocrinology 1997;138:2417-2424
Navot D, Anderson TL, Droesch K, Scott RT, Kreiner D, Rosenwaks Z: Hormonal manipulation of endometrial preparation. J Clin Endocrinol Metab 1989;68:801-807
Russell JB, Knezevich KM, Fabian KF, Dickson JA: Unstimulated immature oocyte retrieval: Early versus midfollicular endometrial priming. Fertil Steril 1997;67:616-620
Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, Tiglias J, Wood C, Trounson AO: Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod 1995;10:3243-3247