Article (Scientific journals)
Two new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide.
Fanuel, L; Thamm, Iris; Kostanjevecki, V et al.
1999In Cellular and Molecular Life Sciences, 55 (5), p. 812-8
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Keywords :
Amino Acid Sequence; Aminopeptidases/genetics/isolation & purification/metabolism; Aniline Compounds; Bacterial Proteins; Base Sequence; Cloning, Molecular; DNA, Bacterial/genetics; Genes, Bacterial; Molecular Sequence Data; Rhizobiaceae/enzymology/genetics; Substrate Specificity
Abstract :
[en] Two new enzymes which hydrolyse D-alanyl-p-nitroanilide have been detected in Ochrobactrum anthropi LMG7991 extracts. The first enzyme, DmpB, was purified to homogeneity and found to be homologous to the Dap protein produced by O. anthropi SCRC C1-38 (ATCC49237). The second enzyme, DmpA, exhibits a similar substrate profile when tested on p-nitroanilide derivatives of glycine and L/D-alanine, but the amounts produced by the Ochrobactrum strain were not sufficient to allow complete purification. Interestingly, the DmpA preparation also exhibited an L-aminopeptidase activity on the tripeptide L-Ala-Gly-Gly but it was not possible to be certain that the same protein was responsible for both p-nitroanilide and peptide hydrolysing activities. The gene encoding the DmpA protein was cloned and sequenced. The deduced protein sequence exhibits varying degrees of similarity with those corresponding to several open reading frames found in the genomes of other prokaryotic organisms, including Mycobacteria. None of these gene products has been isolated or characterised, but a tentative relationship can be proposed with the NylC amidase from Flavobacterium sp. K172.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Fanuel, L
Thamm, Iris ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Kostanjevecki, V
Samyn, B
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Goffin, Colette ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Brannigan, J
Van Beeumen, J
Frère, Jean-Marie ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Two new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide.
Publication date :
1999
Journal title :
Cellular and Molecular Life Sciences
ISSN :
1420-682X
eISSN :
1420-9071
Publisher :
Birkhäuser, Basel, Switzerland
Volume :
55
Issue :
5
Pages :
812-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 June 2012

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