UV Spectroscopy of DNA Duplex and Quadruplex Structures in the Gas Phase

[en] UV absorption spectroscopy is one of the most widely used methods to monitor nucleic acid folding in solution, but the absorption readout is the weighted average contribution of all species present in solution. Mass spectrometry, on the other hand, is able to separate constituents of the solution based on their mass, but methods to probe the structure of each constituent are needed. Here, we explored whether gas-phase UV spectroscopy can give an indication of DNA folding in ions isolated by electrospray mass spectrometry. Model DNA single strands, duplexes, and G-quadruplexes were extracted from solution by electrospray; the anions were stored in a quadrupole ion trap and irradiated by a tunable laser to obtain the UV action spectra of each complex. We found that the duplex and quadruplex spectra are significantly different from the spectra of single strands, thereby suggesting that electronic spectroscopy can be used to probe the DNA gas-phase structure and obtain information about the intrinsic properties of high-order DNA structure.

Disciplines :

Chemistry

Author, co-author :

Rosu, Frédéric ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Gabelica, Valérie ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Antoine, Rodolphe

Broyer, Michel

Dugourd, Philippe

Language :

English

Title :

UV Spectroscopy of DNA Duplex and Quadruplex Structures in the Gas Phase

Publication date :

May 2012

Journal title :

Journal of Physical Chemistry. A

ISSN :

1089-5639

eISSN :

1520-5215

Publisher :

ACS Publications

Volume :

116

Pages :

5383−5391

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 June 2012

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