Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics

Collodoro, Mike; Lemaire, Pascale; Eppe, Gauthier; Bertrand, Virginie; Dobson, Rowan; Mazzucchelli, Gabriel; Widart, Joëlle; De Pauw, Edwin; De Pauw-Gillet, Marie-Claire

doi:10.1016/j.jprot.2012.04.032

Article (Scientific journals)

Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics

Collodoro, Mike; Lemaire, Pascale; Eppe, Gauthier et al.

2012 • In Journal of Proteomics, in press

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/124038

DOI
10.1016/j.jprot.2012.04.032

PubMed
22580036

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Keywords :

17β-estradiol; MCF-7 BOS; 2-D DIGE; Label-free; SRM; xenoestrogens

Abstract :

[en] This paper reports the identification of biomarkers resulting from the exposure of MCF-7/BOS cells to 17β-estradiol (E2). The biomarkers were identified using 2 independent and complementary techniques, 2-D DIGE / MALDI-TOF peptide mass fingerprint, and 2-D UPLC-ESI MS/MS. These markers form a preliminary molecular signature that can be used when testing the estrogenic activity of xenobiotics, either pure or in mixtures.

Research Center/Unit :

Giga-Systems Biology and Chemical Biology - ULiège
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège

Disciplines :

Food science
Environmental sciences & ecology
Chemistry

Author, co-author :

Collodoro, Mike ; Université de Liège - ULiège > Doct. sc. (bioch., biol. mol.&cell., bioinf.&mod.-Bologne)

Lemaire, Pascale ; Université de Liège - ULiège > Doct. sc. (bioch., biol. mol.&cell., bioinf.&mod.-Bologne)

Eppe, Gauthier ; Université de Liège - ULiège > Chimie analytique inorganique

Bertrand, Virginie ; Université de Liège - ULiège > Doct. sc. (chimie - Bologne)

Dobson, Rowan ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Mazzucchelli, Gabriel ; Université de Liège - ULiège > Center for Analytical Research and Technology (CART)

Widart, Joëlle ; Université de Liège - ULiège > Département de pharmacie > Département de pharmacie

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie

Language :

English

Title :

Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17β-estradiol by 2-D DIGE and label-free proteomics

Publication date :

02 May 2012

Journal title :

Journal of Proteomics

ISSN :

1874-3919

eISSN :

1876-7737

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

in press

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.sciencedirect.com/science/article/pii/S1874391912002515

Funders :

FEDER - Fonds Européen de Développement Régional
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
UE - Union Européenne

Available on ORBi :

since 31 May 2012

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