NF-kappa B; CIKS/Act1; NEMO/IKK gamma; signal transduction
Abstract :
[en] Nuclear factor kappaB (NF-kappaB) plays a pivotal role in numerous cellular processes, including stress response, inflammation, and protection from apoptosis. Therefore, the activity of NF-kappaB needs to be tightly regulated. We have previously identified a novel gene, named CIKS (connection to IkappaB-kinase and SAPK), able to bind the regulatory sub-unit NEMO/IKKgamma and to activate NF-kappaB. Here, we demonstrate that CIKS forms homo-oligomers, interacts with NEMO/IKKgamma, and is recruited to the IKK-complex upon cell stimulation. In addition, we identified the regions of CIKS responsible for these functions. We found that the ability of CIKS to oligomerize, and to be recruited to the IKK-complex is not sufficient to activate the NF-kappaB In fact, a deletion mutant of CIKS able to oligomerize, to interact with NEMO/IKKgamma, and to be recruited to the IKK-complex does not activate NF-kappaB, suggesting that CIKS needs a second level of regulation to efficiently activate NF-kappaB. (C) 2003 Elsevier Inc. All rights reserved.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mauro, Claudio; ‘Federico II’ University of Naples > Dipartimento di Biologia e Patologia Cellulare e Molecolare
Vito, Pasquale; Universita degli Studi del Sannio (Benevento) > Dipartimento di Scienze Biologiche e Ambientali
Mellone, Stefano; CNR (Naples) > Istituto di Endocrinologia e Oncologia Sperimentale
Pacifico, Francesco; CNR (Naples) > Istituto di Endocrinologia e Oncologia Sperimentale
Chariot, Alain ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Formisano, Silvestro; ‘Federico II’ University of Naples > Dipartimento di Biologia e Patologia Cellulare e Molecolare
Leonardi, Antonio; ‘Federico II’ University of Naples > Dipartimento di Biologia e Patologia Cellulare e Molecolare
Language :
English
Title :
Role of the adaptor protein CIKS in the activation of the IKK complex
Publication date :
12 September 2003
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press Inc Elsevier Science, San Diego, United States - California
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