[en] Three new insertion elements, IS Mbov1, ISMbov2 and ISMbov3, which are closely related to ISMag1 (Mycoplasma agalactiae), ISMmy1 and IS1634 (both Mycoplasma in mycoides subsp. in mycoides SC), respectively, have been discovered in Mycoplasma bovis, an important pathogen of cattle. Southern blotting showed that the genome of M. bovis harbours 6-12 copies of ISMbov1, 11-15 copies of ISMbov2 and 4-10 copies of ISMbov3, depending on the strain. A fourth insertion element, the IS30-like element, is present in 4-8 copies. This high number of IS elements in AI bovis, which represent a substantial part of its genome, and their relatedness with IS elements of both M. agalactiae and M. mycoides subsp. mycoides SC suggest the occurrence of two evolutionary events: (i) a divergent evolution into ill. agalactiae and M. bovis upon infection of different hosts; (ii) a horizontal transfer of IS elements during co-infection with M. mycoides subsp. mycoides SC and Ad. bovis of a same bovine host. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Disciplines :
Microbiology
Author, co-author :
Thomas, Anne
Linden, Annick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage
Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Bischof, D. F.
Frey, Joachim
Vilei, Edi
Language :
English
Title :
Mycoplasma bovis shares insertion sequences with Mycoplasma agalactiae and Mycoplasma mycoides subsp mycoides SC: Evolutionary and developmental aspects
D.S. Adegboye, P.G. Halbur, D.L. Cavanaugh, R.E. Werdin, C.C.L. Chase, D.W. Miskimins, and R.F. Rosenbusch Immunohistochemical and pathological study of Mycoplasma bovis-associated lung abscesses in calves J. Vet. Diagn. Invest. 7 1995 333 337
C.C. Pinnow, J.A. Butler, K. Sachse, H. Hotzel, L.L. Timms, and R.F. Rosenbusch Detection of Mycoplasma bovis in preservative-treated field milk samples J. Dairy Sci. 84 2001 1640 1645
A.P. Burnens, P. Bonnemain, U. Bruderer, L. Schalch, L. Audige, D. Le Grand, F. Poumarat, and J. Nicolet The seroprevalence of Mycoplasma bovis in lactating cows in Switzerland, particularly in the Republic and Canton of Jura Schweiz. Arch. Tierh. 141 1999 455 460
N. Brice, D. Finlay, D.G. Bryson, J. Henderson, W. Mcconnell, and H.J. Ball Isolation of Mycoplasma bovis from cattle in Northern Ireland, 1993 to 1998 Vet. Rec. 146 2000 643 644
W.J. Byrne, R. McCormack, N. Brice, J. Egan, B. Markey, and H.J. Ball Isolation of Mycoplasma bovis from bovine clinical samples in the Republic of Ireland Vet. Rec. 148 2001 331 333
L.J. Kusiluka, B. Ojeniyi, and N.F. Friis Increasing prevalence of Mycoplasma bovis in Danish cattle Acta Vet. Scand. 41 2000 139 146
A. Thomas, H. Ball, I. Dizier, A. Trolin, C. Bell, J. Mainil, and A. Linden Isolation of mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in Belgium Vet. Rec. 151 2002 472 476
B. Bhugra, and K. Dybvig Identification and characterization of IS1138, a transposable element from Mycoplasma pulmonis that belongs to the IS3 family Mol. Microbiol. 7 1993 577 584
J. Frey, X. Cheng, P. Kuhnert, and J. Nicolet Identification and characterization of IS1296 in Mycoplasma mycoides subsp. mycoides SC and presence in related mycoplasmas Gene 160 1995 95 100
M.J. Calcutt, J.L. Lavrrar, and K.S. Wise IS1630 of Mycoplasma fermentans, a novel IS30-type insertion element that targets and duplicates inverted repeats of variable length and sequence during insertion J. Bacteriol. 181 1999 7597 7607
E.M. Vilei, J. Nicolet, and J. Frey IS1634, a novel insertion element creating long, variable-length direct repeats which is specific for Mycoplasma mycoides subsp. mycoides small-colony type J. Bacteriol. 181 1999 1319 1323
J. Westberg, A. Persson, B. Pettersson, M. Uhlén, and K.-E. Johansson ISMmy1, a novel insertion sequence of Mycoplasma mycoides subsp. mycoides small colony type FEMS Microbiol. Lett. 208 2002 207 213
P. Pilo, B. Fleury, M. Marenda, J. Frey, and E.M. Vilei Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae Vet. Microbiol. 92 2003 37 48
I. Lysnyansky, Y. Ron, K. Sachse, and D. Yogev Intrachromosomal recombination within the vsp locus of Mycoplasma bovis generates a chimeric variable surface lipoprotein antigen Infect. Immun. 69 2001 3703 3712
Y. Ron, R. Flitman-Tene, K. Dybvig, and D. Yogev Identification and characterization of a site-specific tyrosine recombinase within the variable loci of Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae Gene 292 2002 205 211
I. Lysnyansky, Y. Ron, and D. Yogev Juxtaposition of an active promoter to vsp genes via site-specific DNA inversions generates antigenic variation in Mycoplasma bovis J. Bacteriol. 183 2001 5698 5708
H.J. Ball, D. Finlay, and G.A. Reilly Sandwich ELISA detection of Mycoplasma bovis in pneumonic calf lungs and nasal swabs Vet. Rec. 135 1994 531 532
C.J. Su, S.F. Dallo, and J.B. Baseman Molecular distinctions among clinical isolates of Mycoplasma pneumoniae J. Clin. Microbiol. 28 1990 1538 1540
S. Subramaniam, D. Bergonier, F. Poumarat, S. Capaul, Y. Schlatter, J. Nicolet, and J. Frey Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR Mol. Cell. Probes 12 1998 161 169
F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl Current protocols in molecular biology 1999 Wiley New York
L. Falquet, M. Pagni, P. Bucher, N. Hulo, C.J.A. Sigrist, K. Hofmann, and A. Bairoch The PROSITE database, its status in 2002 Nucleic Acids Res. 30 2002 235 238
C. De Meirsman, C. Van Soom, C. Verreth, A. Van Gool, and J. Vanderleyden Nucleotide sequence analysis of IS427 and its target sites in Agrobacterium tumefaciens T37 Plasmid 24 1990 227 234
J. Mahillon, and M. Chandler Insertion sequences Microbiol. Mol. Biol. Rev. 62 1998 725 774
W.S. Hu, M.M. Hayes, R.Y. Wang, J.W. Shih, and S.C. Lo High-frequency DNA rearrangements in the chromosomes of clinically isolated Mycoplasma fermentans Curr. Microbiol. 37 1998 1 5
X. Cheng, J. Nicolet, F. Poumarat, J. Regalla, F. Thiaucourt, and J. Frey Insertion element IS1296 in Mycoplasma mycoides subsp. mycoides small colony identifies a European clonal line distinct from African and Australian strains Microbiology 141 1995 3221 3228
D. Pitcher, and J. Hilbocus Variability in the distribution and composition of insertion sequence-like elements in strains of Mycoplasma fermentans FEMS Microbiol. Lett. 160 1998 101 109
B. Pettersson, M. Uhlen, and K.E. Johansson Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group Int. J. Syst. Bacteriol. 46 1996 1093 1098
M.H. Königsson, G. Bölske, and K.E. Johansson Intraspecific variation in the 16S rRNA gene sequences of Mycoplasma agalactiae and Mycoplasma bovis strains Vet. Microbiol. 85 2002 209 220
A. Thomas, I. Dizier, A. Linden, J. Mainil, J. Frey, and E.M. Vilei Conservation of the uvrC gene sequence in Mycoplasma bovis and its use in routine PCR diagnosis Vet. J. 168 2004 100 102
S. Tola, G. Idini, A.M. Rocchigiani, D. Manunta, P.P. Angioi, S. Rocca, M. Cocco, and G. Leori Comparison of restriction pattern polymorphism of Mycoplasma agalactiae and Mycoplasma bovis by pulsed field gel electrophoresis Zentralbl. Veterinarmed. [B] 46 1999 199 206
A. Thomas, K. Sachse, I. Dizier, C. Grajetzki, F. Farnir, J.G. Mainil, and A. Linden Adherence to various host cell lines of Mycoplasma bovis strains differing in pathogenic and cultural features Vet. Microbiol. 91 2003 101 113