[en] OBJECTIVES: To evaluate the predictive value of RHD fetal genotype in maternal plasma of Rh D negative mothers after 10 weeks of gestation in a clinical use. MATERIAL AND METHOD: Prospective, comparative study between fetal RHD genotyping in maternal plasma, with amplification of exons 4,5,10 of the RHD gene, by real-time multiplex PCR, and Rh D serology at birth, in 218 pregnancy and their 223 babies, between November 2002 and 2004. RESULTS: Combining the amplification of three exons, the concordance rate of fetal Rh D genotyping in maternal plasma and baby phenotyping at delivery was 100%. Four women whose the babies were Rh D negative were positive for RHD exon 10 during pregnancy. This positivity was, in three cases, correlated with the presence of RHDpsi pseudogene and in last case, with a haplotype Cdes (r's). RHD genotyping was performed for five twin pregnancies. CONCLUSION: Multiplex PCR using maternal plasma provides perfect prenatal prediction of fetal RHD gene. These results confirm that this non invasive procedure is the preferred method for assessing Rh D fetal status in Rh negative mothers. Using this method for two years in routine practice has led us to modify our management scheme for sensitized Rh D-negative pregnant women.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Minon, Jean-Marc
Schaaps, Jean-Pierre ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Embryologie
Retz, Maria Christina
Dricot, Jean-François
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Senterre, Jean-Marc
Language :
French
Title :
Utilisation en routine clinique du genotypage foetal RHD sur plasma maternel: bilan de deux ans d'activite
Alternative titles :
[en] Prenatal Determination of Fetal Rhd in Maternal Plasma: Two-Years Experience of Routine Clinical Use
Publication date :
September 2005
Journal title :
Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
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