HPLC profile and dynamic surface properties of the proteose-peptone fraction from bovine milk and from whey protein concentrate

Innocente, Nadia; Biasutti, Marialuisa; Blecker, Christophe

doi:10.1016/j.idairyj.2010.11.004

Article (Scientific journals)

HPLC profile and dynamic surface properties of the proteose-peptone fraction from bovine milk and from whey protein concentrate

Innocente, Nadia; Biasutti, Marialuisa; Blecker, Christophe

2011 • In International Dairy Journal, 21 (4), p. 222-228

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/119977

DOI
10.1016/j.idairyj.2010.11.004

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Keywords :

FAT GLOBULE-MEMBRANE; EMULSIFYING PROPERTIES; HYDROPHOBIC FRACTION; PROTEOLYSIS; CASEINS

Abstract :

[en] Extraction of proteose-peptones (PPs) was carried out from fresh milk, from milk after prolonged incubation (12 days at 37 degrees C) and from two different whey protein concentrates (WPCs). The high performance liquid chromatography profiles of these extracts were compared. PPs eluted as three chromatographic peaks, which increased during milk incubation. The PP extracts from WPCs showed a number of peaks that are attributable to small peptides belonging to the proteose-peptone fraction and probably derive from proteolysis during storage of WPC. The dynamic properties at the air-water interface of the PP extracts were investigated by the drop-volume method. The PPs extracted from fresh milk showed the lowest values and a more rapid reduction in surface tension. PPs from WPCs were found to be effective as surfactants, even though a less marked reduction in surface tension compared with PPs from milk samples was shown. (C) 2011 Elsevier Ltd. All rights reserved.

Disciplines :

Food science

Author, co-author :

Innocente, Nadia

Biasutti, Marialuisa

Blecker, Christophe

Language :

English

Title :

HPLC profile and dynamic surface properties of the proteose-peptone fraction from bovine milk and from whey protein concentrate

Publication date :

April 2011

Journal title :

International Dairy Journal

ISSN :

0958-6946

Publisher :

Elsevier Sci Ltd, Oxford, United Kingdom

Volume :

Issue :

Pages :

222-228

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 May 2012

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