HPLC profile and dynamic surface properties of the proteose-peptone fraction from bovine milk and from whey protein concentrate

Innocente, Nadia; Biasutti, Marialuisa; Blecker, Christophe

doi:10.1016/j.idairyj.2010.11.004

Article (Scientific journals)

HPLC profile and dynamic surface properties of the proteose-peptone fraction from bovine milk and from whey protein concentrate

Innocente, Nadia; Biasutti, Marialuisa; Blecker, Christophe

2011 • In International Dairy Journal, 21 (4), p. 222-228

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/119977

DOI
10.1016/j.idairyj.2010.11.004

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Keywords :

FAT GLOBULE-MEMBRANE; EMULSIFYING PROPERTIES; HYDROPHOBIC FRACTION; PROTEOLYSIS; CASEINS

Abstract :

[en] Extraction of proteose-peptones (PPs) was carried out from fresh milk, from milk after prolonged incubation (12 days at 37 degrees C) and from two different whey protein concentrates (WPCs). The high performance liquid chromatography profiles of these extracts were compared. PPs eluted as three chromatographic peaks, which increased during milk incubation. The PP extracts from WPCs showed a number of peaks that are attributable to small peptides belonging to the proteose-peptone fraction and probably derive from proteolysis during storage of WPC. The dynamic properties at the air-water interface of the PP extracts were investigated by the drop-volume method. The PPs extracted from fresh milk showed the lowest values and a more rapid reduction in surface tension. PPs from WPCs were found to be effective as surfactants, even though a less marked reduction in surface tension compared with PPs from milk samples was shown. (C) 2011 Elsevier Ltd. All rights reserved.

Disciplines :

Food science

Author, co-author :

Innocente, Nadia

Biasutti, Marialuisa

Blecker, Christophe

Language :

English

Title :

HPLC profile and dynamic surface properties of the proteose-peptone fraction from bovine milk and from whey protein concentrate

Publication date :

April 2011

Journal title :

International Dairy Journal

ISSN :

0958-6946

Publisher :

Elsevier Sci Ltd, Oxford, United Kingdom

Volume :

Issue :

Pages :

222-228

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 May 2012

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Bibliography

Andrews A.T. The formation and structure of some proteose-peptone components. Journal of Dairy Research 1979, 46:215-218.
Andrews A.T. Proteinases in normal bovine milk and their action on caseins. Journal of Dairy Research 1983, 50:45-55.
Andrews A.T., Alichanidis A. Proteolysis of caseins and the proteose-peptone fraction of bovine milk. Journal of Dairy Research 1983, 50:275-290.
Official methods of analysis. Nitrogen in milk (Kjeldahl methods)-method 920.20, ash of milk (gravimetric method)-method 945.46, non-protein nitrogen content of milk (Kjeldahl method)-method 991.22 1995, A.O.A.C. (Association of Official Analytical Chemists), AOAC Int, Arlington, VA, USA. 16th ed.
Aschaffenburg R., Drewry J. New procedure for the routine determination of the various non-casein proteins of milk. XVth International Dairy Congress 1959, 3:1631-1637.
Bastian E.D., Brown R.J. Plasmin in milk and dairy products: an update. International Dairy Journal 1996, 6:437-457.
De Noni, I. (2002). Technological and analytical aspects related to shelf life of fresh pasteurized milk. In: " 7th workshop on the developments in the Italian PhD research in food science and technology" Alghero, Italy. September 19-21, pp. 41-48.
De Noni I., Pellegrino L., Cattaneo S., Resmini P. HPLC of proteose-peptones for evaluating ageing of packaged pasteurised milk. International Dairy Journal 2007, 17:12-19.
Girardet J.M., Linden G. PP3 component of bovine milk: a phosphorylated whey glycoprotein. Journal of Dairy Research 1996, 63:333-350.
Grieve P.A., Kitchen B. Proteolysis in milk: the significance of proteinases originating from milk leucocytes and a comparison of the action of leucocyte, bacterial and natural milk proteinases on casein. Journal of Dairy Research 1985, 52:101-112.
Dried milk, dried whey, dried buttermilk and dried butter serum. Determination of fat content (Röse Gottlieb reference method) 1987, IDF, IDF standards 9C, International Dairy Federation, Brussels, Belgium.
Determination of water content of milk powder 1993, IDF, IDF standards 26A, International Dairy Federation, Brussels, Belgium.
Innocente N., Comparin D., Corradini C. Proteose-peptone whey fraction as emulsifier in ice-cream preparation. International Dairy Journal 2002, 12:69-74.
Innocente N., Corradini C., Blecker C., Paquot M. Dynamic surface properties of the proteose-peptone fraction of bovine milk. Journal of Dairy Science 1998, 81:1833-1838.
Innocente N., Corradini C., Blecker C., Paquot M. Emulsifying properties of the total fraction and the hydrophobic fraction of bovine milk proteose-peptones. International Dairy Journal 1998, 8:981-985.
Jeon I.J., Galitzer S.J., Hennessy K.J. Rapid determination of lactose and its hydrolyzates in whey and whey permeate by high performance liquid chromatography. Journal of Dairy Science 1984, 67:884-887.
Kester J.J., Brunner J.R. Milk-fat globule membrane as possible origin of proteose-peptone glycoproteins. Journal of Dairy Science 1982, 65:2241-2252.
Nejjar Y., Pâquet D., Godbillon G., Le Deaut J.Y. Immunological relationship between the hydrophobic fraction of proteose-peptone and the milk fat globule membrane of bovine milk. International Journal of Biochemistry 1986, 18:893-900.
Pâquet D. Review: the proteose-peptone fraction of milk. Le Lait 1989, 69:1-21.
Pâquet D., Driou A., Bracquard P., Linden G. Effect of refrigerated storage of milk on proteolysis. Relationship to the proteose-peptone content. Netherlands Milk and Dairy Journal 1987, 41:81-92.
Pâquet D., Nejjar Y., Alais C. Electrophoretic and chromatographic behavior of the proteose-peptone fraction of cow's milk. Milchwissenschaft 1985, 40:200-203.
Pâquet D., Nejjar Y., Linden G. Study of a hydrophobic protein fraction isolated from milk proteose-peptone. Journal of Dairy Research 1988, 71:1464-1471.
Schaar J. Plasmin activity and proteose-peptone content of individual milk. Journal of Dairy Research 1985, 52:369-378.
Shimizu M., Yamauchi K., Saito M. Emulsifying properties of the proteose-peptone fraction obtained from bovine milk. Milchwissenschaft 1989, 44:497-500.
Sørensen E.S., Petersen E.T. Purification and characterization of three proteins isolated from the proteose-peptone fraction of bovine milk. Journal of Dairy Research 1993, 60:189-197.
Tornberg E. Review: the application of the drop volume technique to measurements of the adsorption of proteins at interfaces. Journal of Colloid and Interface Science 1977, 3:391-402.
Vanderghem D., Danthine S., Blecker C., Deroanne C. Effect of proteose-peptone addition on some physico-chemical characteristics of recombined dairy creams. International Dairy Journal 2007, 17:889-895.