Article (Scientific journals)
PTV-LV-GC/MS/MS as screening and complementary method to HRMS for the monitoring of dioxin levels in food and feed
Eppe, Gauthier; Focant, Jean-François; Pirard, Catherine et al.
2004In Talanta, 63 (5), p. 1135-1146
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Keywords :
programmable temperature vaporization injector; large volume injection; tandem mass spectrometry; polychlorinated dibenzo-p-dioxin; dibenzofuran; food; feed
Abstract :
[en] Recent developments in trapping efficiency inside ion trap mass spectrometer permitted to lower instrument detection limit (IDL). An IDL of 200 fg mul(-1) injected with a signal-to-noise ratio of 5:1 for tetrachlorodibenzo-p-dioxin (TCDD) was obtained by gas chromatography coupled to a quadrupole ion storage mass spectrometer in tandem mode (GC/MS/MS). Coupling large volume programmable temperature vaporizer (PTV-LV) injection to GC/MS/MS provides an alternative and complementary method to classical splitless-GC injection connected to high-resolution mass spectrometry (splitless-GC/HRMS) method for dioxin monitoring in food and feed. An injection volume of 10 mul was found to be the best compromise between the sensitivity requirements and the robustness required for a high throughput method. PTV-LV-GC/MS/MS and Splitless-GC/HRMS were compared by performing analysis on five different matrices such as beef fat, yolk eggs, milk powder, animal feed and serum samples covering a concentration range of two orders of magnitude (i.e. 0.2-25 ng WHO-TEQ kg(-1)). An analysis of variance (ANOVA) was carried out. Fisher tests pointed out that the method effect for all the 2,3,7,8 congeners was not significant, indicating that the null hypothesis (H-0: mu(1) = mu(2) = ... = mu(n)) was not rejected. Moreover, the interaction effects between methods and matrices were not significant for most of the 2,3,7,8 congeners. However, three congeners (2,3,7,8-TCDF; 1,2,3,4,7,8-HxCDD and I,2,3,4,6,7,8-HpCDD) were characterized by P-values lower than the significance level (alpha = 0.05). In toxic equivalence (TEQ), the study showed that no significant bias was observed between the two methods. Consequently, PTV-LV-GC/MS/MS is an attractive technique and can be used as a cost effective complementary method to HRMS for dioxin levels monitoring in food and feed. (C) 2004 Elsevier B.V. All rights reserved.
Disciplines :
Chemistry
Author, co-author :
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de Chimie (Sciences) > Laboratoire de Spectrométrie de masse, Centre interfac. d'analyse des résidus en traces (CART)
Focant, Jean-François  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Pirard, Catherine  
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de Chimie (Sciences) > Laboratoire de Spectrométrie de masse
Language :
English
Title :
PTV-LV-GC/MS/MS as screening and complementary method to HRMS for the monitoring of dioxin levels in food and feed
Publication date :
08 August 2004
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
63
Issue :
5
Pages :
1135-1146
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 April 2009

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