Peroxisomal and mitochondrial status of two murine oligodendrocytic cell lines (158N, 158JP): potential models for the study of peroxisomal disorders associated with dysmyelination processes
[en] In some neurodegenerative disorders (leukodystrophies) characterized by myelin alterations, the defect of peroxisomal functions on myelin-producing cells (oligodendrocytes) are poorly understood. The development of in vitro models is fundamental to understanding the physiopathogenesis of these diseases. We characterized two immortalized murine oligodendrocyte cell lines: a normal (158N) and a jimpy (158JP) cell line mutated for the proteolipid protein PLP/DM20. Fluorescence microscopy, flow cytometry, and western blotting analysis allow to identify major myelin proteins (PLP colocalizing with mitochondria; myelin basic protein), oligodendrocyte (CNPase and myelin oligodendrocyte glycoprotein), and peroxisomal markers [adrenoleukodystrophy protein, PMP70, acyl-CoA oxidase 1 (ACOX1), l-peroxisomal bifunctional enzyme, and catalase]. Using electron microscopy, peroxisomes were identified in the two cell lines. Gene expression (ATP-binding cassette, Abcd1, Abcd2, Abcd3, and Acox1) involved in peroxisomal transport or beta-oxidation of fatty acids was evaluated using quantitative PCR. 4-phenylbutyrate treatment increases expression of ACOX1, l-peroxisomal bifunctional enzyme, PLP, myelin oligodendrocyte glycoprotein, and CNPase, mainly in 158N cells. In both cell lines, 4-phenylbutyrate-induced ACOX1 and catalase activities while only Abcd2 gene was up-regulated in 158JP. Moreover, the higher mitochondrial activity and content observed in 158JP were associated with higher glutathione content and increased basal production of reactive oxygen species revealing different redox statuses. Altogether, 158N and 158JP cells will permit studying the relationships between peroxisomal defects, mitochondrial activity, and oligodendrocyte functions.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Baarine, Mauhamad; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Ragot, Kevin; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Genin, Emmanuelle ; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
El Hajj, Hammam; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Trompier, Doriane; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Andreoletti, Pierre; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Ghandour, Said; UMR CNRS/ULP-Université Louis Pasteur, Strasbourg (FRANCE) > Biopathologie et Imagerie de la Myeline > Laboratoire d’Imagerie et de Neurosciences Cognitives
Menetrier, Franck; IFR Sante/STIC INSERM > Centre de Microscopie Préparative Appliqué à la Biologie et à la Médecine, Dijon (FRANCE)
Cherkaoui-Malki, Mustapha; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Savary, Stéphane; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Lizard, Gérard; INSERM UMR866-Université de Bourgogne, Dijon (FRANCE) > Centre de Recherche Lipides, Nutrition, Cancer > Laboratoire de Biochimie Métabolique et Nutritionnelle
Language :
English
Title :
Peroxisomal and mitochondrial status of two murine oligodendrocytic cell lines (158N, 158JP): potential models for the study of peroxisomal disorders associated with dysmyelination processes
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