Influence of electrical properties on the evaluation of the surface hydrophobicity of Bacillus subtilis

[en] The surface hydrophobicity of nine Bacillus subtilis strains in different states (spores, vegetative cells, and dead cells) was assessed by water contact angle measurements, hydrophobic interaction chromatography (HIC) and bacterial adhesion to hydrocarbon (BATH). Electrokinetic properties of B, subtilis strains were characterized by zeta potential measurements and found to differ appreciably according to the strain, Correlations between HIC data, BATH data and zeta potential showed that HIC and RATH are influenced by electrostatic interactions. Water contact angle measurements thus provide a better estimate of cell surface hydrophobicity. The water contact angle of B. subtilis varied according to the strain and the state, the spores tending to be more hydrophobic than vegetative cells. (C) 2001 Elsevier Science B.V. All rights reserved.

Disciplines :

Agriculture & agronomy

Author, co-author :

Ahimou, F.

Paquot, Michel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech

Jacques, Philippe

Thonart, Philippe ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles

Rouxhet, P. G.

Language :

English

Title :

Influence of electrical properties on the evaluation of the surface hydrophobicity of Bacillus subtilis

Publication date :

2001

Journal title :

Journal of Microbiological Methods

ISSN :

0167-7012

eISSN :

1872-8359

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

Issue :

Pages :

119-126

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 03 May 2010

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