Article (Scientific journals)
Interactions of the antifungal mycosubtilin with ergosterol-containing interfacial monolayers
Nasir, Mehmet Nail; Besson, Françoise
2012In Biochimica et Biophysica Acta. Biomembranes, 1818 (5), p. 1302-1318
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Abstract :
[en] Mycosubtilin, an antimicrobial lipopeptide produced by Bacillus subtilis, is characterized by strong antifungal activities. The molecular mechanisms of its biological activities on the membranes of the sensitive yeasts or fungi have not yet been clearly elucidated. Our purpose was to mimic the mycosubtilin interactions with these membranes using various Langmuir monolayers. Since the major sterol of yeasts or fungi is ergosterol, the interactions of mycosubtilin with monolayers constituted by ergosterol, DPPC/ergosterol or DPPC/sphingomyelin/ergosterol were examined at different initial surface pressures (Πi). Plotting the mycosubtilin-induced surface pressure increases versus Πi allowed to determine that the exclusion pressures of mycosubtilin from these different monolayers is higher than the surface prevailing within the biological membranes. However, this behavior was lost when mycosubtilin was interacting with ergosteryl acetate-containing monolayers. This suggests the involvement of the sterol alcohol group in the mycosubtilin interactions within membranes. Furthermore, the behavior of mycosubtilin with stigmasterol, similar to that observed with ergosterol, differs from that previously observed with cholesterol, suggesting a role of the alkyl side chain of the sterols. The adsorption of mycosubtilin to ergosterol monolayers induced changes in the lipopeptide orientation at the air-water interface as revealed by polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS). Moreover, imaging the air-water interface by Brewster angle microscopy (BAM) indicates that mycosubtilin induced changes in the organization and morphology of monolayers containing pure ergosterol with the appearance of small condensed dots, suggesting again that the target of mycosubtilin might be the ergosterol present in the membranes of the sensitive yeasts or fungi.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Nasir, Mehmet Nail ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Besson, Françoise
Language :
English
Title :
Interactions of the antifungal mycosubtilin with ergosterol-containing interfacial monolayers
Publication date :
2012
Journal title :
Biochimica et Biophysica Acta. Biomembranes
ISSN :
0005-2736
Publisher :
Elsevier Science
Volume :
1818
Issue :
5
Pages :
1302-1318
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 March 2012

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