Investigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds

[en] REASONS FOR PERFORMING STUDY: There is increasing evidence that the equine athlete is exposed to exercise-induced changes of its oxidant/antioxidant balance and antioxidant supplementation is frequently recommended. However, it is unknown whether there is a specific need for antioxidants according to performance, breed, gender or age. OBJECTIVES: To assess whether breed-, gender- and age-related differences of blood oxidant/antioxidant markers occur in competition horses. METHODS: Healthy horses (n = 493) underwent oxidant/ antioxidant blood marker determination. Vitamin E, lipophilic antioxidant capacity (ACL), ascorbic acid (AA), glutathione (GSH, GSSG), gluthione peroxidase (GPx), superoxide dismutase (SOD), selenium (Se), copper (Cu), zinc (Zn), lipid peroxides (Pool), oxidised proteins (Protox) were determined, as well as magnesium (Mg), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), packed cell volume (PCV) and haemoglobin (Hb). A mixed linear model assessed the effect of breed, gender and age category. P<0.05 was considered significant. RESULTS: Thoroughbreds showed the highest values of vitamin E, ACL, GPx, PCV and Hb, whilst standardbreds had the highest values of AA and LDH. Jumping horses had the highest Protox values. Females had significantly higher SOD values, whereas most of the other markers were higher in stallions and geldings. Horses age 2-6 years had higher AA, SOD and LDH values than horses age >6 years. Correlation analyses were positive and significant between vitamin E and GPx, VitE and ACL, Se and GPx, Cu and Pool and negative between Pool and vitamin E, Pool and ACL, Protox and GPx, Protox and vitamin E. CONCLUSIONS: Blood oxidant/ antioxidant status of horses is influenced by breed, gender and age. The correlation analyses suggest synergistic relations between GPx, vitamin E and Se and an antagonistic relation between Protox-GPx, Protox-vitamin E, and Pool-vitamin E. POTENTIAL RELEVANCE: The results of this investigation provide definition of the specific need for antioxidants and vitamins in competition horses.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology
Veterinary medicine & animal health

Author, co-author :

Kirschvink, Nathalie

de Moffarts, Brieuc

Farnir, Frédéric ; Université de Liège - ULiège > Département de productions animales > Biostatistique, économie, sélection animale

Pincemail, Joël

Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie

Language :

English

Title :

Investigation of blood oxidant/antioxidant markers in healthy competition horses of different breeds

Publication date :

2006

Journal title :

Equine Veterinary Journal

ISSN :

0425-1644

eISSN :

2042-3306

Publisher :

Equine Veterinary Journal Ltd, Newmarket Suffolk, United Kingdom

Volume :

Pages :

239-244

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 March 2012

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