Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay

[en] Objectives: Validation of the Architect 25-OH vitamin D assay. Design and methods: Determination of repeatability, reproducibility, accuracy profile and 25(OH)-vitamin D2 recovery on native samples. Comparison with DiaSorin Liaison and RIA. Results and conclusion: Coefficients of variation: b6% (13.6 ng/mL) and 2.2% (78.1 ng/mL). Functional sensitivity: 5 ng/mL. Accuracy profile shows that the method is validated between 13.6 and 78.1 ng/mL. Recovery of 25(OH)D2: 75,8%( 95% CI: 61.9–89.7%). Good correlation with DiaSorin RIA and Liaison b50 ng/mL; above this threshold a systematic positive bias was observed.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

CAVALIER, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

CARLISI, Ignazia ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

BEKAERT, Anne-Catherine ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

ROUSSELLE, Olivier ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

Chapelle, Jean-Paul ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Souberbielle, Jean-Claude

Language :

English

Title :

Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay

Publication date :

2012

Journal title :

Clinical Biochemistry

ISSN :

0009-9120

eISSN :

1873-2933

Publisher :

Elsevier Science, Tarrytown, United States - New York

Volume :

Pages :

505-508

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 February 2012

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Bibliography

Heaney R.P. Vitamin D: how much do we need, and how much is too much?. Osteoporos Int 2000, 11:553-555.
Cavalier E., Delanaye P., Chapelle J.P., Souberbielle J.C. Vitamin D: current status and perspectives. Clin Chem Lab Med 2009, 47:120-127.
Wielders J.P., Wijnberg F.A. Preanalytical stability of 25(OH)-vitamin D3 in human blood or serum at room temperature: solid as a rock. Clin Chem 2009, 55:1584-1585.
EURACHEM/CITAC Guide CG 4 Quantifying uncertainty in analytical measurement 2000, Ref Type: Patent. Second edition.
International Organization for Standardization (ISO) ISO/DTS 21748 Guide to the use of repeatability, reproducibility and trueness estimates in measurement uncertainty estimation 2003, Geneva. Ref Type: Patent.
Cavalier E., Wallace A.M., Carlisi A., Chapelle J.P., Delanaye P., Souberbielle J.C. Cross-reactivity of 25-hydroxy vitamin D2 from different commercial immunoassays for 25-hydroxy vitamin D: an evaluation without spiked samples. Clin Chem Lab Med 2011, 49:555-558.
Cavalier E., Ferir A.M., Delanaye P., Krzesinski J.M., Chapelle J.P. Measurement uncertainty of creatinine in low values: another good reason not to use the MDRD formula with low creatinine values. Clin Biochem 2007, 40:285-286.
Carter G.D., Jones J.C., Berry J.L. The anomalous behaviour of exogenous 25-hydroxyvitamin D in competitive binding assays. J Steroid Biochem Mol Biol 2007, 103:480-482.
Cavalier E., Wallace A.M., Knox S., Mistretta V.I., Cormier C., Souberbielle J.C. Serum vitamin D measurement may not reflect what you give to your patients. J Bone Miner Res 2008, 23:1864.
Cavalier E., Huberty V., Cormier C., Souberbielle J.C. Overestimation of the 25OHD serum concentration with the automated IDS EIA kit. J Bone Miner Res 2011, 26:434-436.