Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay

[en] Objectives: Validation of the Architect 25-OH vitamin D assay. Design and methods: Determination of repeatability, reproducibility, accuracy profile and 25(OH)-vitamin D2 recovery on native samples. Comparison with DiaSorin Liaison and RIA. Results and conclusion: Coefficients of variation: b6% (13.6 ng/mL) and 2.2% (78.1 ng/mL). Functional sensitivity: 5 ng/mL. Accuracy profile shows that the method is validated between 13.6 and 78.1 ng/mL. Recovery of 25(OH)D2: 75,8%( 95% CI: 61.9–89.7%). Good correlation with DiaSorin RIA and Liaison b50 ng/mL; above this threshold a systematic positive bias was observed.

Disciplines :

Laboratory medicine & medical technology

Author, co-author :

CAVALIER, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

CARLISI, Ignazia ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

BEKAERT, Anne-Catherine ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

ROUSSELLE, Olivier ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

Chapelle, Jean-Paul ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Souberbielle, Jean-Claude

Language :

English

Title :

Analytical evaluation of the new Abbott Architect 25-OH vitamin D assay

Publication date :

2012

Journal title :

Clinical Biochemistry

ISSN :

0009-9120

eISSN :

1873-2933

Publisher :

Elsevier Science, Tarrytown, United States - New York

Volume :

Pages :

505-508

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 February 2012

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Bibliography

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