[en] OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development.
METHODS AND RESULTS:
In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates.
CONCLUSIONS:
These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Geudens, I.
Herpers, R.
Hermans, K.
Segura, I.
Ruiz de Almodovar, Carine
Bussmann, J.
De Smet, F.
Vandevelde, W.
Hogan, B. M.
Siekmann, A.
Claes, F.
Moore, J. C.
Pistocchi, A. S.
Loges, S.
Mazzone, M.
Mariggi, G.
Bruyère, F.
Cotelli, F.
Kerjaschki, D.
Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
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