Upregulation of MMPs by soluble E-cadherin in human lung tumor cells

[en] Loss of E-cadherin/catenin mediated cell-cell adhesion and overexpression of matrix metalloproteinases (MMPs) are largely involved in tumor invasion. It has been recently shown that high levels of a soluble 80 kDa fragment of E-cadherin, resulting from a cleavage by IVIMPs, are found in serum and in urine from cancer patients. Additionally, this soluble E-cadherin (sE-CAD) promotes cell invasion into chick heart and into collagen type I gels. The aim of our study was to examine the mechanism of sE-CAD-induced cell invasion. Since MMPs play a crucial role in invasion, we looked for induction of MMPs by sE-CAD in noninvasive human lung tumor cells 16HBE. An induction of MMP-2, MMP-9 and MTI-MMP expression was observed both at the mRNA and at the protein level in the presence of sE-CAD (in conditioned medium form or in E-cadherin HAV peptide form). No induction of MMP-I, -3 and -7 or variation of the levels of their inhibitors, TIMP-I and TIMP-2, were detected. The biologic relevance of the sE-CAD-induced MMP upregulation was tested by demonstrating that sE-CAD promotes in vitro cell invasion in a modified Boyden chamber assay. These data provide new insight into mechanisms of tumor invasion by ectodomain shedding of the cell-cell adhesion molecule E-cadherin. (C) 2003 Wiley-Liss, Inc.

Disciplines :

Oncology

Author, co-author :

Nawrocki-Raby, B.

Gilles, Christine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Polette, M.

Bruynfeel, E.

Laronze, J. Y.

Bonnet, N.

Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique

Mareel, M.

Birembaut, P.

Language :

English

Title :

Upregulation of MMPs by soluble E-cadherin in human lung tumor cells

Publication date :

20 July 2003

Journal title :

International Journal of Cancer

ISSN :

0020-7136

eISSN :

1097-0215

Publisher :

Wiley Liss, Inc., New York, United States - New York

Volume :

105

Issue :

Pages :

790-795

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 April 2009

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104

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