Reference : Nitrated proteins in bronchoalveolar lavage fluid of patients at risk of ventilator-a...
Scientific journals : Article
Human health sciences : Anesthesia & intensive care
Nitrated proteins in bronchoalveolar lavage fluid of patients at risk of ventilator-associated bronchopneumonia.
Mathy, Marianne [Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Damas, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Nys, Monique mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs - Anesthésie et réanimation >]
Deby, Ginette [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Canivet, Jean-Luc mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Ledoux, Didier mailto [Centre Hospitalier Universitaire de Liège - CHU > > Soins intensifs >]
Lamy, Maurice mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anesthésie et réanimation >]
European Respiratory Journal
European Respiratory Society
Yes (verified by ORBi)
[en] Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bronchoalveolar Lavage Fluid/chemistry ; Bronchopneumonia/etiology/metabolism ; Cell Survival ; Cells, Cultured ; Epithelial Cells/physiology ; Female ; Humans ; Male ; Middle Aged ; Neutrophils/enzymology/physiology ; Nitrates/metabolism ; Pancreatic Elastase/metabolism ; Peroxidase/metabolism ; Proteins/metabolism ; Pulmonary Alveoli/cytology/physiology ; Reference Values ; Respiration, Artificial/adverse effects ; Respiratory Distress Syndrome, Adult/metabolism ; Risk Factors
[en] The study was designed to identify markers of oxidative injury, related to the nitric oxide derived cascade, in bronchoalveolar lavage (BAL) fluid from intensive care patients suspected of ventilator-associated pneumonia (VAP) and/or acute respiratory distress syndrome (ARDS). Thirty-eight patients developing VAP and/or ARDS (VAP/ARDS group) were compared to 20 ventilated patients without VAP/ARDS (control group). Myeloperoxidase (MPO) and elastase, taken as markers of neutrophil activation were measured by enzymatic techniques, and nitrated proteins (NTPs) by an immunological method. The cytotoxicity of the BAL fluid was tested using cultured human epithelial alveolar cells by the release of pre-incorporated 51Cr. Mean NTP concentration and, MPO and elastase activities were different between the VAP/ARDS and control groups (p<0.05 for NTPs; p<0.005 for MPO; p<0.005 for elastase). NTP concentration correlated with MPO and elastase activity and neutrophil number (r=0.93, 0.91 and 0.87, respectively), but not to protein concentration and arterial oxygen tension/inspiratory oxygen fraction. The cytotoxicity of BAL correlated with NTP concentration (r=0.92) and MPO activity (r=0.89). It was concluded that the concentrations of nitrated proteins in bronchoalveolar lavage fluid correlated with the oxidant activity of neutrophils and that, bronchoalveolar lavage fluid cytotoxicity was correlated with the nitrated protein concentration and may be mediated by oxidants.

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