Reference : Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact ...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Screening for a low-cost Haematococcus pluvialis medium reveals an unexpected impact of a low N:P ratio on vegetative growth
Tocquin, Pierre* mailto [Université de Liège - ULiège > Département des sciences de la vie > labo de Bioénergétique > >]
Fratamico, Anthony* mailto [Université de Liège - ULiège > > Labo de Bioénergétique >]
Franck, Fabrice mailto [Université de Liège - ULiège > > Labo de Bioénergétique >]
* These authors have contributed equally to this work.
Journal of Applied Phycology
Yes (verified by ORBi)
[en] Haematococcus pluvialis ; Culture media optimization ; Phosphate supply ; Nutrient induced fluorescence transients
[en] Haematococcus pluvialis is the current better source of natural astaxanthin, a high-value carotenoid.
Traditionally, the production process of astaxanthin by this algae is achieved by a two-stage system:
during the first stage, vegetative “green” cells are produced and then converted, in the second stage, into
cysts that accumulate astaxanthin. In this work, a medium screening strategy based on the mixing of a
3-component hydroponic fertilizer was applied to identify a new formulation optimized for the vegetative
stage. A maximal and high cell density of 2 x 106 cells mL−1 was obtained in a medium containing
a high level of phosphate relative to nitrate, resulting in a N:P ratio much lower than commonly used
media for H. pluvialis. In this medium, cells remained at the vegetative and motile stage during a
prolonged period of time. Both high cell density culture and motile stage persistence was proved to
be related to the N:P feature of this medium. We conclude that the macrozoid stage of H. pluvialis
is favored under high-P and low-N supply and that low-cost hydroponic fertilizers can be successfully
used for achieving high density cultures of vegetative cells of H. pluvialis.
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Energie
Researchers ; Professionals ; Students

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