Reference : In vitro study of the antioxidant properties of non steroidal anti-inflammatory drugs...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
In vitro study of the antioxidant properties of non steroidal anti-inflammatory drugs by chemiluminescence and electron spin resonance (ESR).
Mouithys-Mickalad, Ange mailto [Université de Liège - ULiège > > Centre de l'oxygène : Recherche et développement (C.O.R.D.)]
Zheng, S. X. [> > > >]
Deby-Dupont, G. P. [> > > >]
Deby, C. [ > > ]
Lamy, Maurice mailto [Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques >]
Reginster, Jean-Yves mailto [Université de Liège - ULiège > Département des sciences de la santé publique > Santé publique, Epidémiologie et Economie de la santé]
Henrotin, Yves mailto [Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)]
Free Radical Research
Taylor & Francis
Yes (verified by ORBi)
United Kingdom
[en] Anti-Inflammatory Agents, Non-Steroidal/metabolism/pharmacology ; Antioxidants/metabolism/pharmacology ; Ascorbic Acid ; Chemiluminescent Measurements ; Chlorine/metabolism ; Diclofenac/analogs & derivatives/metabolism/pharmacology ; Electron Spin Resonance Spectroscopy ; Free Radicals/metabolism ; Humans ; Indicators and Reagents ; Indomethacin/metabolism/pharmacology ; Iron ; Kinetics ; Lipid Peroxidation ; Neutrophil Activation ; Neutrophils/drug effects/metabolism ; Nitrates/metabolism ; Reactive Oxygen Species/metabolism ; Sodium Hypochlorite/pharmacology ; Spectrophotometry, Ultraviolet ; Sulfonamides/metabolism/pharmacology ; Tetradecanoylphorbol Acetate
[en] OBJECTIVES: To determine the antioxidant activities of nonsteroidal anti-inflammatory drugs (NSAIDS), we examined by chemiluminescence (CL) and electron spin resonance (ESR) their scavenging properties towards lipid peroxides, hypochlorous acid and peroxynitrite. METHODS: The antioxidant properties of nimesulide (NIM), 4-hydroxynimesulide (4-HONIM), aceclofenac (ACLO), 4-hydroxyaceclofenac (4-HOA-CLO), diclofenac (DICLO) and indomethacin (INDO) were tested on four different reactive oxygen species (ROS) generating systems: (I) phorbol-myristate acetate (PMA)-activated neutrophils, (II) Fe2+/ascorbate-induced lipid peroxidation, (III) HOCl-induced light emission, (IV) the kinetics of ONOO- decomposition followed by spectrophotometry. ROS production was monitored by luminol-enhanced CL or by ESR using two different spin traps. RESULTS: At 10 microM, ACLO, NIM, 4-HONIM, 4-HOA-CLO, and DICLO decreased luminol-enhanced CL generated by PMA-activated neutrophils. Inversely, INDO increased the luminol enhanced CL. Interestingly, hydroxylated metabolites were more potent antioxidants than the parent drugs. Furthermore, all drugs tested, excepted ACLO, lowered lipid peroxidation induced by Fe2+/ascorbate system. ACLO and DICLO, even at the highest concentration tested (100 microM), did not significantly lower HOCl induced CL, whereas the other drugs were potent scavengers. Finally, all the NSAIDS accelerated decomposition of ONOO-, suggesting a potential capacity of the molecules to scavenge peroxynitrite. CONCLUSION: The NSAIDs possess variable degrees of antioxidant activities, linked to their ability to react with HOCl, lipid peroxides or ONOO-. These antioxidant activities could offer interesting targeted side-effects in the treatment of joint inflammatory diseases.

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