Sequencing of Bovine herpesvirus 4 V.test strain reveals important genome features

Palmeira, Leonor; Machiels, Bénédicte; Lété, Céline; Vanderplasschen, Alain; Gillet, Laurent

doi:10.1186/1743-422X-8-406

Reference : Sequencing of Bovine herpesvirus 4 V.test strain reveals important genome features


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Multidisciplinary, general & others Life sciences : Veterinary medicine & animal health Life sciences : Genetics & genetic processes
	To cite this reference:	http://hdl.handle.net/2268/103138

Title :	Sequencing of Bovine herpesvirus 4 V.test strain reveals important genome features
Language :	English
Author, co-author :	Palmeira, Leonor [Université de Liège - ULiège > > Immunologie et vaccinologie >]
	Machiels, Bénédicte [Université de Liège - ULiège > > Immunologie et vaccinologie >]
	Lété, Céline [Université de Liège - ULiège > > Immunologie et vaccinologie >]
	Vanderplasschen, Alain [Université de Liège - ULiège > > Immunologie et vaccinologie >]
	Gillet, Laurent [Université de Liège - ULiège > > Immunologie et vaccinologie >]
Publication date :	2011
Journal title :	Virology Journal
Volume :	8
Issue/season :	1
Pages :	406
Peer reviewed :	Yes
Audience :	International
Abstract :	[en] Background Bovine herpesvirus 4 (BoHV-4) is a useful model for the human pathogenic gammaherpesviruses Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus. Although genome manipulations of this virus have been greatly facilitated by the cloning of the BoHV-4 V.test strain as a Bacterial Artificial Chromosome (BAC), the lack of a complete genome sequence for this strain limits its experimental use. Methods In this study, we have determined the complete sequence of BoHV-4 V.test strain by a pyrosequencing approach. Results The long unique coding region (LUR) consists of 108,241 bp encoding at least 79 open reading frames and is flanked by several polyrepetitive DNA units (prDNA). As previously suggested, we showed that the prDNA unit located at the left prDNA-LUR junction (prDNA-G) differs from the other prDNA units (prDNA-inner). Namely, the prDNA-G unit lacks the conserved pac-2 cleavage and packaging signal in its right terminal region. Based on the mechanisms of cleavage and packaging of herpesvirus genomes, this feature implies that only genomes bearing left and right end prDNA units are encapsulated into virions. Conclusions In this study, we have determined the complete genome sequence of the BAC-cloned BoHV-4 V.test strain and identified genome organization features that could be important in other herpesviruses.
Permalink :	http://hdl.handle.net/2268/103138
DOI :	10.1186/1743-422X-8-406
Other URL :	http://www.virologyj.com/content/8/1/406/

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