Sequencing of Bovine herpesvirus 4 V.test strain reveals important genome features

[en] Background Bovine herpesvirus 4 (BoHV-4) is a useful model for the human pathogenic gammaherpesviruses Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus. Although genome manipulations of this virus have been greatly facilitated by the cloning of the BoHV-4 V.test strain as a Bacterial Artificial Chromosome (BAC), the lack of a complete genome sequence for this strain limits its experimental use. Methods In this study, we have determined the complete sequence of BoHV-4 V.test strain by a pyrosequencing approach. Results The long unique coding region (LUR) consists of 108,241 bp encoding at least 79 open reading frames and is flanked by several polyrepetitive DNA units (prDNA). As previously suggested, we showed that the prDNA unit located at the left prDNA-LUR junction (prDNA-G) differs from the other prDNA units (prDNA-inner). Namely, the prDNA-G unit lacks the conserved pac-2 cleavage and packaging signal in its right terminal region. Based on the mechanisms of cleavage and packaging of herpesvirus genomes, this feature implies that only genomes bearing left and right end prDNA units are encapsulated into virions. Conclusions In this study, we have determined the complete genome sequence of the BAC-cloned BoHV-4 V.test strain and identified genome organization features that could be important in other herpesviruses.

Disciplines :

Life sciences: Multidisciplinary, general & others
Veterinary medicine & animal health
Genetics & genetic processes

Author, co-author :

Palmeira, Leonor ; Université de Liège - ULiège > Immunologie et vaccinologie

Machiels, Bénédicte ; Université de Liège - ULiège > Immunologie et vaccinologie

Lété, Céline ; Université de Liège - ULiège > Immunologie et vaccinologie

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie

Language :

English

Title :

Sequencing of Bovine herpesvirus 4 V.test strain reveals important genome features

Publication date :

2011

Journal title :

Virology Journal

eISSN :

1743-422X

Publisher :

BioMed Central, United Kingdom

Volume :

Issue :

Pages :

406

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.virologyj.com/content/8/1/406/

Available on ORBi :

since 15 November 2011

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