Article (Scientific journals)
Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs
Amarger, V.; Nguyen, Minh Ngoc; Van Laere, Anne-Sophie et al.
2002In Mammalian Genome, 13 (7), p. 388-398
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Abstract :
[en] IGF2 is the major candidate gene for a paternally expressed Quantitative Trait Locus (QTL) in the pig primarily affecting muscle development. Here we report two sequence contigs together comprising almost 90 kb containing the INS-IGF2 and H19 genes. A comparative sequence analysis of the pig, human, and mouse genomic sequences was conducted to identify the exon/intros organization, all promoters, and other evolutionarily conserved elements. RT-PCR analysis showed that IGF2 transcripts originated from four different promoters and included various combinations of seven untranslated exons together with three coding exons, in agreement with previous findings in other mammals. The observed sequence similarity in intronic and intragenic regions among the three species is remarkable and is most likely explained by the complicated regulation of imprinting and expression of these genes. The general trend was, as expected, a higher sequence similarity between human and pig than between these species and the mouse, but a few exceptions to this rule were noted. This genomic region exhibits several striking features, including a very high GC content, many CpG islands, and a low amount of interspersed repeats. The high GC and CpG content were more pronounced in the pig than in the two other species. The results will facilitate the further characterization of this important QTL in the pig.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Amarger, V.
Nguyen, Minh Ngoc ;  Université de Liège - ULiège > LTAS - Infographie
Van Laere, Anne-Sophie  ;  Université de Liège - ULiège > Département de productions animales > Génomique animale
Braunschweig, M.
Nezer, Carine ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Georges, Michel  ;  Université de Liège - ULiège > Département de productions animales > Génomique animale
Andersson, L.
Language :
Title :
Comparative sequence analysis of the INS-IGF2-H19 gene cluster in pigs
Publication date :
July 2002
Journal title :
Mammalian Genome
Publisher :
Springer-Verlag, New York, United States - New York
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
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