Construction and characterization of an ovine BAC contig spanning the callipyge locus.

SEGERS, Karin; Vaiman, D.; Berghmans, S.; Shay, T.; Meyers, S.; Beever, J.; Cockett, N.; Charlier, Carole; Georges, Michel

doi:10.1046/j.1365-2052.2000.00676.x

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Construction and characterization of an ovine BAC contig spanning the callipyge locus.

SEGERS, Karin; Vaiman, D.; Berghmans, S. et al.

2000 • In Animal Genetics, 31 (6), p. 352-9

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https://hdl.handle.net/2268/102189

DOI
10.1046/j.1365-2052.2000.00676.x

PubMed
11167521

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Keywords :

Animals; Base Sequence; Chromosomes, Artificial, Bacterial; DNA Primers; Electrophoresis, Gel, Pulsed-Field; Polymerase Chain Reaction; Sequence Tagged Sites; Sheep/genetics

Abstract :

[en] We describe the construction of an ovine BAC contig spanning a 4.6 centimorgan (cM) chromosome segment known to contain the callipyge (CLPG) locus. The contig comprises 21 ovine BAC clones jointly covering approximately 900 kilobases (Kb). Two gaps in the BAC contig, spanning 10 and 7.5 Kb, respectively, were bridged by long range PCR. The corresponding chromosome region was shown to be characterized by an unusually low Kb to cM ratio (164 Kb/cM) and a high density of Not1 sites (1:126 Kb) possibly reflecting a high gene density in the corresponding chromosome region. Equivalent amplification of 64 sequence tagged sites spanning the corresponding region from homozygous +/+ and CLPG/CLPG individuals disproves the hypothesis of a major deletion causing the CLPG mutation.

Disciplines :

Genetics & genetic processes

Author, co-author :

SEGERS, Karin ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Vaiman, D.

Berghmans, S.

Shay, T.

Meyers, S.

Beever, J.

Cockett, N.

Charlier, Carole

Georges, Michel ; Université de Liège - ULiège > Unit of Animal Genomics

Language :

English

Title :

Construction and characterization of an ovine BAC contig spanning the callipyge locus.

Publication date :

2000

Journal title :

Animal Genetics

ISSN :

0268-9146

eISSN :

1365-2052

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

Issue :

Pages :

352-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 09 November 2011

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