Article (Scientific journals)
A 2.5-Mb contig constructed from Angus, Longhorn and horned Hereford DNA spanning the polled interval on bovine chromosome 1
Wunderlich, K. R.; Abbey, C. A.; Clayton, D. R. et al.
2006In Animal Genetics, 37 (6), p. 592-594
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Keywords :
BAC contig; bovine chromosome 1; polled; STS; Animals; Cattle/genetics; Chromosomes, Artificial, Bacterial; Chromosomes, Mammalian; Horns; Contig Mapping/veterinary; Humans; Phenotype
Abstract :
[en] The polled locus has been mapped by genetic linkage analysis to the proximal region of bovine chromosome 1. As an intermediate step in our efforts to identify the polled locus and the underlying causative mutation for the polled phenotype, we have constructed a BAC-based physical map of the interval containing the polled locus. Clones containing genes and markers in the critical interval were isolated from the TAMBT (constructed from Angus and Longhorn genomic DNA) and CHORI-240 (constructed from horned Hereford genomic DNA) BAC libraries and ordered based on fingerprinting and the presence or absence of 80 STS markers. A single contig spanning 2.5 Mb was assembled. Comparison of the physical order of STSs to the corresponding region of human chromosome 21 revealed the same order of genes within the polled critical interval. This contig of overlapping BAC clones from horned and polled breeds is a useful resource for SNP discovery and characterization of positional candidate genes.
Disciplines :
Agriculture & agronomy
Genetics & genetic processes
Zoology
Author, co-author :
Wunderlich, K. R.
Abbey, C. A.
Clayton, D. R.
Song, Y.
Schein, J. E.
Georges, Michel  ;  Université de Liège - ULiège > Département de productions animales > Génomique animale
Coppieters, Wouter ;  Université de Liège - ULiège > Département de productions animales > Département de productions animales
Adelson, D. L.
Taylor, J. F.
Davis, S. L.
Gill, C. A.
Language :
English
Title :
A 2.5-Mb contig constructed from Angus, Longhorn and horned Hereford DNA spanning the polled interval on bovine chromosome 1
Publication date :
December 2006
Journal title :
Animal Genetics
ISSN :
0268-9146
eISSN :
1365-2052
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
37
Issue :
6
Pages :
592-594
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 November 2011

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