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Insertional mutagenesis to select mutants for modified hydrogen photoproduction in Chlamydomonas reinhardtii
Godaux, Damien; Emonds-Alt, Barbara; Cardol, Pierre et al.
2011Microorganisms for Bio-fuel Production from Sunlight
 

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Keywords :
Chlamydomonas; hydrogen; mutagenesis
Abstract :
[en] The unicellular green alga Chlamydomonas reinhardtii has evolved the ability to redirect electrons from the photosynthetic chain to drive hydrogen production via chloroplast oxygen-sensitive hydrogenases. This process occurs under anaerobic conditions and provides a biological basis for solar-driven hydrogen production. Nevertheless, the yield is a major limitation for an economic viability and fundamental knowledge is still needed in order to have a better understanding of the process. In 2000, Melis and co-worker defined a protocol allowing a sustainable hydrogen production in sulfur deprivation condition. By adjustment of an existent protocol called the Winkler test, we are trying to isolate mutants with an attenuated photosynthesis to respiration capacity ratio (P/R ratio). This kind of mutants could be able to reach anoxia needed for hydrogenases activity without the stressful impact of sulfur deprivation. An insertional mutagenesis of Chlamydomonas has been carried out with an hygromycin resistance cassette and about 2500 transformants have generated and screened by the adapted Winkler test. We have isolated several oxygen-consuming mutants and the most promising one is subject to functional, molecular and genetic characterization.
Research center :
ULg - Université de Liège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Godaux, Damien ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Emonds-Alt, Barbara 
Cardol, Pierre  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Franck, Fabrice ;  Université de Liège - ULiège > Labo de Bioénergétique
Remacle, Claire  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Language :
English
Title :
Insertional mutagenesis to select mutants for modified hydrogen photoproduction in Chlamydomonas reinhardtii
Alternative titles :
[en] Mutagenèse insertionelle afin d'isoler des mutants déficients pour la photoproduction d'hydrogène chez chlamydomonas reinhardtii
Publication date :
18 September 2011
Number of pages :
A0
Event name :
Microorganisms for Bio-fuel Production from Sunlight
Event organizer :
European science foundation
Event place :
Bielefeld, Germany
Event date :
18/09/2011-21/09/2011
Audience :
International
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 19 October 2011

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