Azidothymidine as a potential modifier of radiation-reponse

[en] Abstract The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 μM AZT on exponentially growing cells was studied after different exposure times (24, 48 and 72 h). The surviving fraction (clonogenic assay) or metabolic activity (MTT assay) after irradiation of AZT-exposed cells, was compared to unexposed irradiated controls. Flow cytometry was used to assess the cell-cycle effect of pre-exposure of exponentially growing cells to AZT. AZT had a radioprotective effect for all experimental time points as far as WiDr was concerned. For HeLa the effect was significant at 24 h. Cell-cycle analysis showed a significant accumulation in S-phase at 72 h for WiDr. For HeLa there was a significant accumulation in S-phase at 48 h. We conclude that under the reported experimental conditions, AZT as a thymidine analogue seems to reduce the cytotoxic effect of irradiation.

Disciplines :

Hematology
Oncology

Author, co-author :

Copaceanu, M-L

COUCKE, Philippe ; Centre Hospitalier Universitaire Vaudois (CHUV) > Radiothérapie

Cottin, E

Mirimanoff, R-O

Language :

English

Title :

Azidothymidine as a potential modifier of radiation-reponse

Publication date :

1995

Journal title :

Acta Oncologica

ISSN :

0284-186X

eISSN :

1651-226X

Publisher :

Taylor & Francis, Oslo, Norway

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 March 2009

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