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07 September 2011
Article (Scientific journals)
A multicentric evaluation of IDMS-traceable creatinine enzymatic assays
Pieroni, Laurence; DELANAYE, Pierre  ; Boutten, Anne et al.
2011 • In Clinica Chimica Acta, 412, p. 2070-2075
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Keywords :
creatinine; IDMS traceable; standardization
Abstract :
[en] Chronic kidney disease definition is based on glomerular filtration rate (GFR) estimations which are derived from creatinine-based equations. The accuracy of GFR estimation is thus largely dependent of those of serum creatinine assays. International recommendations highlight the need for traceable creatinine assays. The French Society of Clinical Biochemistry conducted a study for measuring accuracy of creatinine enzymatic methods. This evaluation involved 25 clinical laboratories. Creatinine was measured in serum pools ranging from 35.9±0.9 μmol/L to 174.5±3.1 μmol/L (IDMS determination) using 12 creatinine enzymatic methods. For all creatinine values greater than 74.4±1.4 μmol/L, the bias and imprecision did not exceed 5% and 5.9%, respectively. For the lowest value (35.9±0.9 μmol/L), the bias ranged from −1.8 to 9.9% (with one exception). At this level, the imprecision ranged from 1.9 to 7.8%. The true performances of the assays (couples of bias and relative standard deviation), were evaluated using Monte-Carlo simulations. Most of the assays fall within the maximum Total Error of 12% at all concentrations. This study demonstrates substantial improvements in the calibration, traceability and precision of the enzymatic methods, reaching the NKDEP recommendations. Moreover, most of these assays allowed accurate creatinine measurements for creatinine levels lower than 40 μmol/L.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Pieroni, Laurence
DELANAYE, Pierre  ;  Centre Hospitalier Universitaire de Liège - CHU > Néphrologie
Boutten, Anne
Bargnoux, Anne-Sophie
Rozet, Eric ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Delatour, Vincent
Carlier, Marie-Christine
Hanser, Anne-Marie
CAVALIER, Etienne  ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Froissart, Marc
Cristol, Jean-Paul
Language :
English
Title :
A multicentric evaluation of IDMS-traceable creatinine enzymatic assays
Publication date :
2011
Journal title :
Clinica Chimica Acta
ISSN :
0009-8981
eISSN :
1873-3492
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
412
Pages :
2070-2075
Peer reviewed :
Peer Reviewed verified by ORBi

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