Article (Scientific journals)
Corneofungimetry bioassay on Malassezia spp. under ketoconazole and desonide influences.
Pierard, Claudine; Vroome, Valérie; Cauwenbergh, Geert et al.
2005In Skin Pharmacology and Physiology, 18 (2), p. 98-102
Peer Reviewed verified by ORBi
 

Files


Full Text
CORNEOFUNGIMETRY.pdf
Author postprint (192.75 kB)
Request a copy

Copyright 2005 S. Karger AG, Basel.


All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Administration, Topical; Biological Assay/methods; Desonide/administration & dosage/pharmacokinetics/therapeutic use; Double-Blind Method; Drug Combinations; Drug Therapy, Combination; Gels; Humans; Ketoconazole/administration & dosage/pharmacokinetics/therapeutic use; Malassezia/drug effects/growth & development; Mycology/methods; Specimen Handling/methods
Abstract :
[en] BACKGROUND: Glucocorticoids can boost some Malassezia-driven dermatoses. However, both antifungals and topical corticosteroids improve lesions of seborrheic dermatitis. OBJECTIVE: To revisit the topical activity of the antifungal ketoconazole and the corticosteroid desonide on Malassezia growth on human stratum corneum. Material and Methods: The computer-assisted corneofungimetry bioassay was used to compare the growth of M. furfur, M. globosa and M. restricta on human stratum corneum coated with olive oil. Four blinded gel formulations were tested. They contained either 2% ketoconazole, 0.05% desonide or a combination of 2% ketoconazole and 0.05% desonide; one gel was unmedicated. Untreated stratum corneum and specimens coated with a 2% ketoconazole cream were used as negative and positive comparators, respectively. A total of 45 samples (15 M. furfur, 15 M. globosa, and 15 M. restricta) were used for each test formulation in this randomized, double-blind study. RESULTS: The 2% ketoconazole gel and cream and the combination of 2% ketoconazole and 0.05% desonide formulation abated similarly and significantly the M. furfur, M. globosa and M. restricta growth. The 3 species were similarly sensitive to these formulations. By contrast, no significant inhibitory effect was yielded by the 0.05% desonide gel and the vehicle. CONCLUSION: The presence of 0.05% desonide does not impair or improve the Malassezia susceptibility to 2% ketoconazole when growing on lipid-enriched human stratum corneum.
Disciplines :
Dermatology
Author, co-author :
Pierard, Claudine ;  Centre Hospitalier Universitaire de Liège - CHU > Dermatopathologie
Vroome, Valérie;  Barrier Therapeutics, nv, Geel, Belgium
Cauwenbergh, Geert
Pierard, Gérald ;  Centre Hospitalier Universitaire de Liège - CHU > Dermatopathologie
Language :
English
Title :
Corneofungimetry bioassay on Malassezia spp. under ketoconazole and desonide influences.
Publication date :
2005
Journal title :
Skin Pharmacology and Physiology
ISSN :
1660-5527
eISSN :
1660-5535
Publisher :
S. Karger, Basel, Switzerland
Volume :
18
Issue :
2
Pages :
98-102
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 March 2009

Statistics


Number of views
68 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
7
Scopus citations®
without self-citations
4
OpenCitations
 
5

Bibliography


Similar publications



Contact ORBi