Inactivation of alpha(2)-Macroglobulin by Activated Human Polymorphonuclear Leukocytes.

[en] The proteolytic activity of trypsin releases the dye Remazol Brilliant Blue from its high molecular weight substrate, the skin powder (Hide Powder Azure, Sigma), with an increase in absorbance at 595 nm. Active alpha(2)- macroglobulin (80 mug/ml) totally inhibits the proteolytic activity of trypsin (14 mug/ml) by trapping this protease. But after a 20 min incubation of alpha(2)-macroglobulin at 37 degrees C with 2 x 10(6) human polymorphonuclear leukocytes activated by N-formyl-L-methionyl-L-leucyl-L-phenylalanine (10(-7) M) and cytochalasin B (10(-8) M), 100% of trypsin activity was recovered, indicating a total inactivation of alpha(2)-macroglobuHn. Incubation with granulocyte myeloperoxidase also inactivates alpha(2)-macroglobulin. Hypochlorous acid, a by-product of myeloperoxidase activity, at a concentration of 10(-7) M also inactivates alpha(2)-macroglobulin, which indicates that an important cause of alpha(2)-macroglobulin inactivation by activated polymorphonuclear leukocytes could be the activity of myeloperoxidase.

Disciplines :

Anesthesia & intensive care
Human health sciences: Multidisciplinary, general & others

Author, co-author :

Deby, Ginette ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)

Croisier, Jean-Louis ; Université de Liège - ULiège > Département des sciences de la motricité > Kinésithérapie générale et réadaptation

Camus, Gérard

Brumioul, D.

Mathy-Hartert, M.; Université de Liège - ULiège > Département des sciences de la santé publique > Immunohématologie - Transfusion

Sondag, Danièle ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques

Deby, C.

Lamy, Maurice

Language :

English

Title :

Inactivation of alpha(2)-Macroglobulin by Activated Human Polymorphonuclear Leukocytes.

Publication date :

1994

Journal title :

Mediators of Inflammation

ISSN :

0962-9351

eISSN :

1466-1861

Publisher :

Hindawi Publishing Corporation, Sylvania, United States - Ohio

Volume :

Issue :

Pages :

117-23

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 March 2009

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