[en] The 165 rRNA sequence of Trichodesmium sp. strain NlBB 1067 was determined
and used for the construction of a distance tree and bootstrap analysis. The
tree shows that, among the available cyanobacterial 165 rRNA sequences,
Trichodesmium NlBB 1067 has Oscillatoria PCC 7515 as its closest relative,
presenting 94.9% of sequence similarity with the latter strain. This is in
contrast to a difference of 9 mol O/O G + C in mean genomic DNA base
composition between the two organisms. Nevertheless, the genotypic
heterogeneity presented by a number of strains assigned to the genus
Oscillatoria hinders a taxonomic decision on the separate existence of the
genera Trichodesmium and Oscillatoria. The sequence of the internal
transcribed spacer (ITS) between the 165 and 23s rRNA genes was also
determined, as a possible marker to study inter- and intraspecif ic variability.
The ITS contains the genes coding for tRNA1Ie and tRNAAia and its total length is
547 nucleotides. In six out of eight sequenced clones, there is a duplication of
29 nucleotides, surrounding the 5' end of the tRNA1Ie.
Research center :
Université d'Anvers (UIA)
Disciplines :
Microbiology
Author, co-author :
Wilmotte, Annick ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Neefs, Jean-Marc
De Wachter, Rupert
Language :
English
Title :
Evolutionary affiliation of the marine nitrogen-fixing cyanobacterium Trichodesmium sp. strain NlBB 1067, derived by 16s ribosomal RNA sequence analysis
Alternative titles :
[en] Affiliation évolutionnaire de la cyanobactérie marine fixatrice d'azote Trichodesmium sp. souche NIBB 1067
