Article (Scientific journals)
Perturbation of actin dynamics induces NF-kappa B activation in myelomonocytic cells through an NADPH oxidase-dependent pathway
Kustermans, Gaëlle; El Benna, Jamel; Piette, Jacques et al.
2005In Biochemical Journal, 387 (Pt 2), p. 531-540
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Keywords :
actin cytoskeleton; myelomonocytic cells; nuclear factor kappa B (NF-kappa B); reactive oxygen species (ROS)
Abstract :
[en] Although several reports showed the effect of compounds disrupting microtubules on NF-kappa B (nuclear factor kappa B) activation, nothing is known about agents perturbing actin dynamics. In the present study, we have shown that actin cytoskeleton disruption induced by actin-depolymerizing agents such as cytochalasin D and latrunculin B and actin-polymerizing compounds such as jasplakinolide induced NF-kappa B activation in myelomonocytic cells. The transduction pathway involved the I kappa B (inhibitory kappa B) kinase complex and a degradation of I kappa B alpha. We have shown that NF-kappa B activation in response to the perturbation of actin dynamics required reactive oxygen species. as demonstrated by the effect of antioxidants. Actin cytoskeleton disruption by cytochalasin D induced O-2(-) release from human monocytes, through the activation of the NADPH oxidase, as confirmed by the phosphorylation and by the membrane translocation of p47(phox). NF-kappa B activation after actin cytoskeleton disruption could be physiologically relevant during monocyte activation and/or recruitment into injured tissues, where cellular attachment, migration and phagocytosis result in cyclic shifts in cytoskeletal organization and disorganization.
Research center :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kustermans, Gaëlle ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
El Benna, Jamel;  INSERM, Paris, france
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Legrand, Sylvie ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
Language :
English
Title :
Perturbation of actin dynamics induces NF-kappa B activation in myelomonocytic cells through an NADPH oxidase-dependent pathway
Publication date :
15 April 2005
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
387
Issue :
Pt 2
Pages :
531-540
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Modulation of the NF-KappaB activation pathways by the actin cytoskeleton
Funders :
IAP 5/12
Available on ORBi :
since 24 March 2009

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