[en] Many physiopathological events such as phagocytosis, pathogen invasion, cellular adhesion
and chemotaxis governed by actin-based cytoskeleton are often accompanied by nuclear
factor kB (NF-kB) activation and expression of pro-inflammatory genes. In the present study,
we demonstrated that reorganization of actin cytoskeleton induced by Cytochalasin D
(CytD), an actin-polymerization inhibitor, enhanced il-8 gene expression induced by TNFa
and LPS in HL-60 monocyte-like cells. Both transcriptional and post-transcriptional
mechanisms were involved. CytD potentiated NF-kB-mediated transcription induced by
both TNFa and LPS but via different mechanisms. In the case of LPS, the perturbation of actin
dynamics increased the TLR4 levels at the cell membrane and consequently enhanced the
IKK complex activation and NF-kB nuclear translocation. However, the canonical pathway
involving the IKK complex and leading to the NF-kB translocation into the nucleus was not
affected by actin remodelling in the case of TNFa. Interestingly, actin disruption primed p65
phosphorylation induced by TNFa and LPS, on Ser276 and Ser536, respectively, which
suggested actin cytoskeleton could also modulate p65 transactivating activity.
Research Center/Unit :
Giga-Signal Transduction - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Kustermans, Gaëlle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
El Mjiyad, Nadia ; Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Horion, Julie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Jacobs, Nathalie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Piette, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - GIGA-Research
Actin cytoskeleton differentially modulates NF-κB-mediated IL-8 expression in myelomonocytic cells
Publication date :
2008
Journal title :
Biochemical Pharmacology
ISSN :
0006-2952
eISSN :
1873-2968
Publisher :
Elsevier Science, Oxford, United Kingdom
Volume :
76(10)
Pages :
1214-1228
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Modulation of the NF-KappaB activation pathways by the actin cytoskeleton
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Funding text :
This work was supported in part by the Interuniversity Attraction Poles (IAP)5/12 and IAP6/18 programs (Brussels, Belgium), Televie (Brussels,
Belgium) and the National Fund for Scientific Research (FNRS Brussels, Belgium). GK and JH were supported by the IAP program and the Télévie, NEM is research assistant at the University of Liège, SLP and NJ are Research Associate and JP is Research Director of the FNRS.
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