Evidence for a role of accessible galactosyl or mannosyl residues of Fc domain in the in vivo clearance of IgG antibody-coated autologous erythrocytes in the rat
[en] The potential role of accessible galactosyl or mannosyl residues of IgG in the
clearance of IgG-coated autologous red blood cells (IgG-RBCs) by the spleen and
the liver was investigated in the rat using rabbit anti-rat RBC IgG antibody
molecules differing from each other by their capacity to bind in vitro to peanut
agglutinin (PNA) and concanavalin A (Con A), i.e., two lectins that specifically
bind to beta-galactosyl and alpha-mannosyl residues of Fc domain, respectively.
Those IgG molecules [IgG(PNA) or IgG(Con A)] were separated from the starting
anti-RBC IgG antibody batch [IgG(total)] by affinity chromatography on lectin
columns. Each IgG-RBC preparation was labeled with 99mTc, and was reinjected iv
with autologous rat RBCs labeled with 111In to correct for 99mTc present in the
blood contained in each organ. The mean specific spleen uptakes per gram of the
three IgG-RBC preparations increased according to the level of RBC sensitization
but were at least 10 times higher in each instance than the mean specific liver
uptake per gram. Consistent with the clearance curves of IgG(PNA)-RBCs, the mean
specific splenic uptake per gram of those RBCs was significantly increased as
compared to the same parameters determined using either IgG(total)-RBCs or
IgG(Con A)-RBCs. In contrast, the mean specific liver uptakes per gram of
IgG(PNA)-RBCs, of IgG(Con A)RBCs, or of IgG(total)-RBCs were not significantly
different under otherwise identical experimental conditions. The increase in the
splenic removal of IgG(PNA)-RBCs was C3 independent. Furthermore, splenic
macrophages isolated from rats were able to bind in vitro significantly more
IgG(PNA)-RBCs than IgG(total)-RBCs or IgG(Con A)-RBCs. These data therefore
support the concept that, in the rat, the kinetic of removal of IgG-RBCs from the
bloodstream by the Fc receptors of splenic mononuclear phagocytes may vary
according to the nature of accessible carbohydrates located in the Fc domain of
IgG antibody molecules coating the erythrocytes.
Disciplines :
Rheumatology
Author, co-author :
Malaise, Michel ; Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie
Hoyoux, C.
Franchimont, P.
Mahieu, P. R.
Language :
English
Title :
Evidence for a role of accessible galactosyl or mannosyl residues of Fc domain in the in vivo clearance of IgG antibody-coated autologous erythrocytes in the rat
Wright, Silverstein (1986) Overview: The function of receptors in phagocytosis. Handbook of Experimental Immunology: Cellular Immunology , D.M. Weir, 3rd ed., Blackwell, Oxford; 2:41.1-41.14.
Nose, Wigzell (1983) Biological significance of carbohydrate chains on monoclonal antibodies. Proc. Natl. Acad. Sci. USA , 3rd ed.; 80:6632-6636.
Malaise, Franchimont, Gomez, Bouillenne, Mahieu (1987) The spontaneous ability of normal human IgG to inhibit the Fc receptors of normal human monocytes is related to their binding capacity to lectins. Clin. Immunol. Immunopathol. 45:1-16.
Ross, Cain, Lachmann (1985) Membrane complement receptor type three (CR3) has lectin-like properties analogous to bovine conglutinin and functions as a receptor for zymosan and rabbit erythrocytes as well as a receptor for iC3b. J. Immunol. 134:3307-3315.
Frank, Lawley, Hamburger, Brown (1983) Immunoglobulin G Fc receptor mediated clearance in auto-immune disease. Ann. Int. Med. 98:206-218.
Malaise, Mahieu (1985) The Fc-receptor function of human mononuclear phagocyte system: Physiological role, alteration in immune diseases and modulation. Acta Clin. Belg. 40:27-37.
Scheidegger (1955) Une microméthode de l'immunoélectrophorèse. Int. Arch. Allergy Appl. Immunol. 7:103-110.
Laemmli (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
Stanworth, Turner (1986) Immunochemical analysis of immunoglobulins and their subunits. Handbook of Experimental Immunology: Immunochemistry , D.M. Weir, 3rd ed., Blackwell, Oxford; 1:12.1-12.46.
Malaise, Franchimont, Bouillenne, Houssier, Mahieu (1987) Increased concanavalin A-binding capacity of immunoglobulin G purified from sera of patients with rheumatoid arthritis. Clin. Exp. Immunol. 68:543-551.
Sinn, Silvester (1979) Simplified cell labelling with indium111 acetylacetone. Brit. J. Radiol. 52:758-759.
David, Reisfeld (1974) Protein iodination with solid state lactoperoxydase. Biochemistry 13:1014-1021.
Kijlstra, van der Lelij, Knutson, Fleuren, van Es (1978) The influence of phagocytic function on glomerular localization of aggregated IgG in rats. Clin. Exp. Immunol. 32:207-213.
Hughes-Jones (1977) Functional affinity constants of the reaction between 125I-labelled C1q and C1q binders and their use in the measurement of plasma C1q concentrations. Immunology 32:191-198.
Friedman, Netti, Schreiber (1985) Effect of estradiol and steroid analogues on the clearance of immunoglobulin G-coated erythrocytes. J. Clin. Invest. 75:162-167.
Kress, Spiro (1986) Studies on the glycoprotein nature of the thyrotropin receptor: Interaction with lectins and purification of the bovine protein with the use of Bandeiraea (Griffonia) simplicifolia I affinity chromatography. Endocrinology 118:974-979.
Yousaf, Howard, Williams (1986) Studies in the rat of antibody-coated and N-ethylmaleimide-treated erythrocyte clearance by the spleen. Immunology 59:75-79.
Yousaf, Howard, Williams (1988) Studies in the rat of antibody-coated and N-ethylmaleimide-treated erythrocyte clearance by the liver: Effects of immune complex infusion and complement activation. Immunology 64:193-199.
Frank, Schreiber, Atkinson, Jaffe (1977) Pathophysiology of immune hemolytic anemia. Annals of Internal Medicine 87:210-222.
Wiener, Joliffe, Scott, Kumpel, Thompson, Melaned, Hughes-Jones (1988) Differences between the activities of human monoclonal IgG1 and IgG3 anti-D antibodies of the Rh blood group system in their abilities to mediate effector functions of monocyte. Immunology 65:159-163.
Elkon, Ferjencik, Walport, Peters, Lewis, Hughes (1982) Evaluation of heat-damaged and IgG-coated red blood cells for testing reticuloendothelial function. J. Immunol. Methods 55:253-263.
Walport, Peters, Elkon, Pusey, Lavender, Hughes (1985) The splenic extraction ratio of antibody-coated erythrocytes and its response to plasma exchange and pulse methylpredisolone. Clin. Exp. Immunol. 60:465-473.
Kimberly, Salmon, Bussel, Kuntz Crow, Hilgartner (1984) Modulation of mononuclear phagocyte function by intravenous γ-globulins. J. Immunol. 132:745-750.
Van Lenten, Ashwell (1972) The binding of desialylated glycoproteins by plasma membranes of rat liver. J. Biol. Chem. 247:4633-4640.
Winkelhake, Nicholson (1976) Aglycosyl antibody: Effects of exoglycosidase treatments on autochtonous antibody survival time in the circulation. J. Biol. Chem. 251:1074-1080.
Findbloom, Magilavy, Harford, Plotz (1981) Influence of antigen on immune complex behavior in mice. J. Clin. Invest. 68:214-224.