Reference : 15-deoxy-delta12,14-prostaglandin J2 inhibits Bay 11-7085-induced sustained extracell...
Scientific journals : Article
Human health sciences : Rheumatology
15-deoxy-delta12,14-prostaglandin J2 inhibits Bay 11-7085-induced sustained extracellular signal-regulated kinase phosphorylation and apoptosis in human articular chondrocytes and synovial fibroblasts
Relic, Biserka [Université de Liège - ULiège > > Rhumatologie >]
Benoit, Valerie [ > > ]
Franchimont, Nathalie [ > > ]
Ribbens, Clio mailto [Université de Liège - ULiège > > Rhumatologie >]
Kaiser, Marie-Joëlle mailto [Université de Liège - ULiège > > Rhumatologie >]
Gillet, Philippe mailto [Université de Liège - ULiège > > Chirurgie appareil locomoteur >]
Merville, Marie-Paule mailto [Université de Liège - ULiège > Département de pharmacie > Chimie médicale >]
Bours, Vincent mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique générale et humaine >]
Malaise, Michel mailto [Université de Liège - ULiège > Département des sciences cliniques > Rhumatologie >]
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
Yes (verified by ORBi)
[en] 15-deoxy-delta(12,14)-prostaglandin J2 ; Annexin A5 ; Anti-Infective Agents ; BAY 11-7085 ; Coloring Agents ; I-kappa B Proteins ; Immunologic Factors ; Leupeptins ; Multienzyme Complexes ; NF-kappa B ; Nitriles ; Receptors, Cytoplasmic and Nuclear
[en] We have previously shown that nuclear factor-kappaB inhibition by adenovirus
expressing mutated IkappaB-alpha or by proteasome inhibitor increases human
articular chondrocytes sensibility to apoptosis. Moreover, the nuclear
factor-kappaB inhibitor BAY11-7085, a potent anti-inflammatory drug in rat
adjuvant arthritis, is itself a proapoptotic agent for chondrocytes. In this
work, we show that BAY 11-7085 but not the proteasome inhibitor MG-132 induced a
rapid and sustained phosphorylation of extracellular signal-regulated kinases
(ERK1/2) in human articular chondrocytes. The level of ERK1/2 phosphorylation
correlated with BAY 11-7085 concentration and chondrocyte apoptosis.
15-Deoxy-delta(12,14)-prostaglandin J2 (15d-PGJ2) and its precursor prostaglandin
(PG) D2 but not PGE2 and PGF2alpha rescued chondrocytes from BAY 11-7085-induced
apoptosis. 15d-PGJ2 markedly inhibited BAY 11-7085-induced phosphorylation of
ERK1/2. BAY 11-7085 also induced ERK1/2 phosphorylation and apoptosis in human
synovial fibroblasts, and these reactions were down-regulated by 15d-PGJ2.
Further analysis in synovial fibroblasts showed that only molecules that
suppressed BAY 11-7085-induced phosphorylation of ERK1/2 (i.e. 15d-PGJ2, PGD2,
and to a lesser extent, MEK1/2 inhibitor UO126, but not prostaglandins E2 and
F2alpha or peroxisome proliferator-activated receptor-gamma agonist ciglitazone)
were able protect cells from apoptosis. These results suggested that the
antiapoptotic effect of 15d-PGJ2 on chondrocytes and synovial fibroblasts might
involve inhibition of ERK1/2 phosphorylation.
Researchers ; Professionals

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