[en] The cellular and humoral responses as well as the antigen recognition during the acute stage of a Neospora caninum (NC) infection were investigated in non-pregnant ewes. The experimentally infected ewes developed specific lymphoproliferative and humoral responses within 2 weeks post-infection (PI). The magnitude of the cellular response showed large variations between animals. A significant decrease in the proliferative response to Con A mitogen and N. caninum, Toxoplasma gondii (TG) antigens was recorded on day 21 post-infection (PI). The humoral response and the pattern of antigen recognition were similar among infected ewes. Proteins of 44, 42, 40, 39 and 28 kDa were intensively recognized by the infected animals during the experiment. The 42 and 28 kDa antigens should be considered as useful for the diagnostic of N. caninum infection, as the intensity of recognition infection of the other antigens had decreased markedly 8 weeks post-infection. For some antigens a sequential recognition was recorded. The 59, 54 and 38-37 kDa proteins were frequently recognized by infected sera during the first weeks of the infection, but recognition of these antigens was absent or rare at the end of the experiment. These antigens could be related to the acute stage of the infection.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Rettigner, Chantal; Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Lasri, Saadia
De Meerschman, Francois
Focant, Charles ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Immune response and antigen recognition in non-pregnant ewes experimentally infected with Neospora caninum tachyzoites.
Alvarez-Garcia G., Pereira-Bueno J., Gomez-Bautista M., Ortega-Mora L.M. Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. Vet. Parasitol. 107:2002;15-27
Andrianarivo A.G., Barr B.C., Anderson M.L., Rowe J.D., Packham A.E., Sverlow K.W., Conrad P.A. Immune responses in pregnant cattle and bovine fetuses following experimental infection with Neospora caninum. Parasitol. Res. 87:2001;817-825
Barta J.R., Dubey J.P. Characterization of anti-Neospora caninum hyperimmune rabbit serum by Western blot analysis and immunoelectron microscopy. Parasitol. Res. 78:1992;689-694
Bjerkas I., Mohn S.F., Presthus J. Unindentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs. Zeitschr Parasitenkunde. 70:1984;271-274
Bjerkas I., Jenkins M.C., Dubey J.P. Identification and characterization of Neospora caninum tachyzoite antigens useful for diagnosis of neosporosis. Clin. Diagn. Lab. Immunol. 2:1994;214-221
Buxton D., Maley S.W., Thomson K.M., Trees A.J., Innes E.A. Experimental infection of non-pregnant and pregnant sheep with Neospora caninum. J. Comp. Pathol. 117:1997;1-16
Buxton D., Maley S.W., Wright S., Thomson K.M., Rae A.G., Innes E.A. The pathogenesis of experimental neosporosis in pregnant sheep. J. Comp. Pathol. 118:1998;267-279
Buxton D., Wright S., Maley S.W., Rae A.G., Lunden A., Innes E.A. Immunity to experimental neosporosis in pregnant sheep. Parasite Immunol. 23:2001;85-91
De Marez T., Liddell S., Dubey J.P., Jenkins M.C., Gasbarre L. Oral infection of calves with Neospora caninum oocysts from dogs: humoral and cellular immune responses. Int. J. Parasitol. 29:1999;1647-1657
De Meerschman F., Rettigner C., Focant C., Boreux R., Pinset C., Leclipteux T., Losson B. Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells. Vet. Res. 33:2002;159-168
Denkers E.Y., Gazzinelli R.T. Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection. Clin. Microbiol. Rev. 11:1998;569-588
Dubey J.P. Recent advances in Neospora and neosporosis. Vet. Parasitol. 84:1999;349-367
Dubey J.P., Lindsay D.S., Adams D.S., Gay J.M., Baszler T.V., Blagburn B.L., Thulliez P. Serologic responses of cattle and other animals infected with Neospora caninum. Am. J. Vet. Res. 57:1996;329-336
Harkins D., Clements D.N., Maley J., Marks J., Wright S., Esteban I., Innes E.A., Buxton D. Western blot analysis of the responses of ruminants infected with Neospora caninum and with Toxoplasma gondii. J. Comp. Pathol. 119:1998;45-55
Innes E.A., Panton W.R.M., Sanderson A., Thomson K.M., Wastling J.M., Maley S., Buxton D. Induction of CD4+ and CD8+ T cell response in efferent lymph responding to Toxoplasma gondii infection: analysis of phenotype and function. Parasite Immunol. 17:1995;151-160
Innes E.A., Lunden A., Esteban I., Marks J., Wright S., Rae A., Harkins D., Vermeulen A., McKendrick I.J., Buxton D. A previous infection with Toxoplasma gondii does not protect against a challenge with Neospora caninum in pregnant sheep. Parasite Immunol. 23:2001;121-132
Khan I.M., Schwartzman J.D., Fonseka S., Kasper L.H. Neospora caninum: role for immune cytokines in host immunity. Exp. Parasitol. 85:1997;24-34
Long M.T., Baszler T.V. Neutralisation of maternal IL-4 modulates congenital protozoal transmission: comparaison of innate versus acquired immune responses. J. Immunol. 164:2000;4768-4774
Lunden A., Marks J., Maley W.M., Innes E.A. Cellular immune response in cattle experimentally infected with Neospora caninum. Parasite Immunol. 20:1998;519-526
Maley S.W., Buxton D., Thomson K.M., Schriefer C.E.S., Innes E.A. Serological analysis of calves experimentally infected with Neospora caninum: a 1-year study. Vet. Parasitol. 96:2001;1-9
Marcolino P.T., Silva D.A.O., Leser P.G., Camargo M.E., Mineo J.R. Molecular markers in acute and chronic phases of human toxoplasmosis: determination of immunoglobulin G avidity by western blotting. Clin. Diagn. Lab. Immunol. 7:2000;384-389
Nishikawa Y., Claveria F.G., Fujisaki K., Nagasawa H. Studies on serological cross-reaction of Neospora caninum with Toxoplasma gondii and Hammondia heydorni. J. Vet. Med. Sci. 64:2002;161-164
O'Handley R., Liddell S., Parker C., Jenkins M.C., Dubey J.P. Experimental infection of sheep with Neospora caninum oocysts. J. Parasitol. 88:2002;1120-1123
Osawa T., Wastling J., Maley S., Buxton D., Innes E.A. A multiple antigen ELISA to detect Neospora-specific antibodies in bovine sera, bovine fœtal fluids, ovine and caprine sera. Vet. Parasitol. 79:1998;19-34
Ritter D.M., Kerlin R., Sibert G., Brake D. Immune factors influencing the course of infection with Neospora caninum in the murine host. J. Parasitol. 88:2002;271-280
Schares G., Peters M., Wurm R., Bärwald A., Conraths F.J. The efficiency of vertical transmission of Neospora caninum in dairy cattle analysed by serological techniques. Vet. Parasitol. 80:1998;87-98
Seow H.-F. Pathogen interactions with cytokines and host defence: an overview. Vet. Immunol. Immunopathol. 63:1998;139-148
Tanaka T., Hamada T., Inoue N., Nagasawa H., Fujisaki K., Suzuki N., Mikami T. The role of CD4+ or CD8+ T cells in the protective immune response of BALB/c mice to Neospora caninum infection. Vet. Parasitol. 90:2000;183-191
Williams D.J.L., Guy C.S., McGarry J.W., Guy F., Tasker L., Smith R.F., MacEachern K., Cripps P.J., Kelly D.F., Trees A.J. Neospora caninum associated abortion in cattle: the time of experimentally-induced parasitemia during gestation determines foetal survival. Parasitology. 121:2000;347-358