[en] Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal cotyledons and characterized biochemically. The isolation procedure included acid and ammonium sulfate precipitations and anion and cation exchange chromatographies. The isolated PAGs have different NH2-terminal amino acid sequences (RGSXLTILPLRNMRDIVY, ISRVSXLTIHPLRNIMDML, and RGSNLTIHPLRNIRD) and apparent molecular masses (55, 57, and 59 kDa). Each shows several isoforms with different pl values. The three proteins share high sequence identity with each other and with other ovine, bovine, and caprine PAGs. They have not been described previously. The ovPAG-59 sequence differs from the previously identified ovPAG-4 sequence (determined by DNA cloning and sequencing) at only one position among the 15 N-terminal residues. The newly characterized ovPAGs and the procedure used to isolate them will be helpful in producing new antisera for investigating PAG secretion in pregnant ewes.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
El Amiri, B.; Université de Liège - ULiège
Remy, Benoit
Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Otthiers, Nicole ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Perenyi, Z.
Mboko, H. B.
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta
Publication date :
2003
Journal title :
Molecular Reproduction and Development
ISSN :
1040-452X
eISSN :
1098-2795
Publisher :
Wiley Liss, Inc., New York, United States - New York
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