Development and validation of a specific radioimmunoassay for equine osteocalcin

[en] This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard, tracer, and immunogen for antibody formation in rabbits. The assay allowed to measure equine serum OC levels with a sensitivity of 0.2 ng/mL. Immunoreactive serum OC values of clinically normal, different-aged horses ranged from 3.68 to 127.31 ng/mL. Intra- and inter-assay coefficients of variation (CV) were 6.2 and 8.2%, respectively. Serial equine scrum sample dilutions were linear. The recovery of equine OC from equine serum samples ranged from 93.88 to 107.9%. There was a tight correlation between OC values measured with the equine- specific OC RIA and two commercially available bovine-specific OC RIA kits. However, highest serum OC values were obtained with the equine-specific OC RIA. In conclusion, our equine-specific OC RIA is sensitive, linear, accurate, precise, and reproducible. The assay allowed to quantify OC in equine serum samples and might, therefore, be used to monitor equine osteoblast activity associated with bone diseases, exercise, therapy forms or diet.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Carstanjen, Bianca

Sulon, Joseph ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Banga-Mboko, H.; Université de Liège - ULiège

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Remy, Benoit; Université de Liège - ULiège > Physiologie de la reproduction

Language :

English

Title :

Development and validation of a specific radioimmunoassay for equine osteocalcin

Publication date :

2003

Journal title :

Domestic Animal Endocrinology

ISSN :

0739-7240

eISSN :

1879-0054

Publisher :

Elsevier Science Inc, New York, United States - New York

Volume :

Issue :

Pages :

31-41

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 March 2009

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