[en] Antigen(s) immunologically related to pregnancy-associated glycoproteins (PAGs) have previously been detected in the serum of pregnant goats. In this work, we describe a partial characterization of a family of PAGs isolated from the placenta of the goat. The procedure, monitored by RIA, included extraction of proteins at neutral pH, acidic, and ammonium sulfate precipitations; and gel filtration and ion exchange chromatographies. Immunoreactivity, initially located in the acidic supernatant and in the 40-80% ammonium sulfate fractions, was equally apportioned between the 0.04 and 0.08 M NaCl DEAE fractions. After further purification of both DEAE fractions, the preparations were subjected to one- and two-dimensional electrophoresis, and individual polypeptides were analyzed by amino acid sequencing. Three PAGs, which differed in amino acid sequence and apparent molecular masses (62, 59, and 55 kDa), were detected, each containing several isoforms with different pls: caprine (c) PAG62 (pl: 5.1, 4.8), cPAG59 (pl: 6.2, 5.9, 5.6), and cPAG55 (pl: 5.3, 5.1, 4.9). These proteins had high sequence identities to each other and to PAGs purified from other species. Each had two putative N-glycosylation sites within the 27 amino terminal residues sequenced. This work demonstrates that PAGs are present in goat placenta and that multiple forms are expressed.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Garbayo, Juana Maria; Université de Liège - ULiège - ULG > Faculté de Médecine Vétérinaire > Physiologie de la Reproduction
Remy, Benoit; Université de Liège - ULiège - ULG > Faculté de Médecine Vétérinaire > Physiologie de la Reproduction
Alabart, José Luis; Agricultural Research Service, Aragón, Spain > Dept of Animal Production
Folch, José; Agricultural Research Service, Aragón, Spain > Dept of Animal Production
Wattiez, Ruddy; University of Mons-Hainaut, Belgium > Dept of Biological Chemistry - School of Sciences
Falmagne, P.
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Isolation and Partial Characterization of a Pregnancy-Associated Glycoprotein Family from the Goat Placenta
Publication date :
1998
Journal title :
Biology of Reproduction
ISSN :
0006-3363
eISSN :
1529-7268
Publisher :
Society for the Study of Reproduction, Madison, United States - Wisconsin
Volume :
58
Issue :
1
Pages :
109-115
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Spanish Ministry of Agriculture, Fisheries and Food IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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