Reference : Multiple pregnancy-associated glycoproteins are secreted by day 100 sheep placenta
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/9188
Multiple pregnancy-associated glycoproteins are secreted by day 100 sheep placenta
English
Xie, Sancai [University of Missouri - MU > Dept of Animal Science > > >]
Green, Jonathan [University of Missouri - MU > Dept of Biochemistry > > >]
Bao, Bagna [University of Missouri - MU > Dept of Animal Science > > >]
Beckers, Jean-François mailto [Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Valdez, Kelli E [University of Missouri - MU > Dept of Animal Science > > >]
Hakami, Livija [University of Missouri - MU > Dept of Veterinary Pathology > > >]
Roberts, R. Michael [University of Missouri - MU > Dept of Biochemistry > > >]
1997
Biology of Reproduction
Society for the Study of Reproduction
57
6
1384-1393
Yes (verified by ORBi)
International
0006-3363
1529-7268
Madison
WI
[en] PAG ; Ovine ; Placenta
[en] Pregnancy-associated glycoprotein (PAG)-1 (PAG1) and pregnancy-specific protein B are either identical or closely related antigens released by trophoblast binucleate cells of placentas of cattle. Sheep and other ruminants produce similar products. There is evidence, however, that these antigens, which are related structurally to the pepsinogens and other aspartic proteinases, are not single gene products but members of an extensive family. Here, the sequential use of ammonium sulfate precipitation and Sepharose blue, anion-exchange, and cation-exchange chromatographies, as well as isoelectric elution from a Mono P column, has allowed several PAG1-related molecules to be purified from the medium after culture of explants from Day 100 sheep placentas. Each of these PAGs cross-reacted to a varying extent with a panel of three different anti-PAG1 antisera. Four of them, all of which were major secretory products of the placenta, were subjected to amino-terminal microsequencing. Although each was related to ovine (ov) PAG1, none was identical. Reverse transcription-polymerase chain reaction was then used to amplify PAG1-related cDNA from Day 100 placental RNA. Seven novel full-length cDNA, all distinct from ovPAG1, were identified from 25 cDNA selected for sequencing. Only two of these (ovPAG3 and ovPAG7) encoded polypeptides identical in sequence at their inferred amino termini to one of the PAGs (ovPAG65) purified from explant cultures. Even so, they were only 84% identical in overall sequence. The remaining five cDNA were unique. In situ hybridization analysis revealed that expression of ovPAG3 and ovPAG7, like that of ovPAG1, is confined to trophoblast binucleate cells. The data confirm that at Day 100 of pregnancy the ovine placenta produces many different PAGs, which differ considerably in sequence and immunological cross-reactivity.
NIH and USDA grant 96-35203-3257
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/9188
10.1095/biolreprod57.6.1384

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