Article (Scientific journals)
Multiple pregnancy-associated glycoproteins are secreted by day 100 sheep placenta
Xie, Sancai; Green, Jonathan; Bao, Bagna et al.
1997In Biology of Reproduction, 57 (6), p. 1384-1393
Peer Reviewed verified by ORBi
 

Files


Full Text
XieS_BiolReprod_1997.pdf
Publisher postprint (1.34 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
PAG; Ovine; Placenta
Abstract :
[en] Pregnancy-associated glycoprotein (PAG)-1 (PAG1) and pregnancy-specific protein B are either identical or closely related antigens released by trophoblast binucleate cells of placentas of cattle. Sheep and other ruminants produce similar products. There is evidence, however, that these antigens, which are related structurally to the pepsinogens and other aspartic proteinases, are not single gene products but members of an extensive family. Here, the sequential use of ammonium sulfate precipitation and Sepharose blue, anion-exchange, and cation-exchange chromatographies, as well as isoelectric elution from a Mono P column, has allowed several PAG1-related molecules to be purified from the medium after culture of explants from Day 100 sheep placentas. Each of these PAGs cross-reacted to a varying extent with a panel of three different anti-PAG1 antisera. Four of them, all of which were major secretory products of the placenta, were subjected to amino-terminal microsequencing. Although each was related to ovine (ov) PAG1, none was identical. Reverse transcription-polymerase chain reaction was then used to amplify PAG1-related cDNA from Day 100 placental RNA. Seven novel full-length cDNA, all distinct from ovPAG1, were identified from 25 cDNA selected for sequencing. Only two of these (ovPAG3 and ovPAG7) encoded polypeptides identical in sequence at their inferred amino termini to one of the PAGs (ovPAG65) purified from explant cultures. Even so, they were only 84% identical in overall sequence. The remaining five cDNA were unique. In situ hybridization analysis revealed that expression of ovPAG3 and ovPAG7, like that of ovPAG1, is confined to trophoblast binucleate cells. The data confirm that at Day 100 of pregnancy the ovine placenta produces many different PAGs, which differ considerably in sequence and immunological cross-reactivity.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Xie, Sancai;  University of Missouri - MU > Dept of Animal Science
Green, Jonathan;  University of Missouri - MU > Dept of Biochemistry
Bao, Bagna;  University of Missouri - MU > Dept of Animal Science
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Valdez, Kelli E;  University of Missouri - MU > Dept of Animal Science
Hakami, Livija;  University of Missouri - MU > Dept of Veterinary Pathology
Roberts, R. Michael;  University of Missouri - MU > Dept of Biochemistry
Language :
English
Title :
Multiple pregnancy-associated glycoproteins are secreted by day 100 sheep placenta
Publication date :
1997
Journal title :
Biology of Reproduction
ISSN :
0006-3363
eISSN :
1529-7268
Publisher :
Society for the Study of Reproduction, Madison, United States - Wisconsin
Volume :
57
Issue :
6
Pages :
1384-1393
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NIH and USDA grant 96-35203-3257
Available on ORBi :
since 18 March 2009

Statistics


Number of views
78 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
60
Scopus citations®
without self-citations
20
OpenCitations
 
49

Bibliography


Similar publications



Contact ORBi