Anticorps anti-PMSG chez la brebis soumise à un traitement annuel de synchronisation des chaleurs. Les conséquences des traitements annuels de synchronisation des oestrus sur les performances de reproduction des brebis ont été étudiées dans neuf troupeaux Lacaune de l’Aveyron (France) adhérant au contrôle laitier officiel. Un traitement hormonal, associant la pose d’une éponge vaginale imprégnée de fluorogestone acétate (FGA) pendant 14 j suivi de l’injection de PMSG (-500 UI) a été appliqué sur les brebis de sept troupeaux, les brebis des deux autres élevages, mises en lutte sur oestrus naturel ont servi de témoins. Des échantillons sanguins ont été prélevés juste avant l’injection de PMSG (pour tester la présence d’anticorps résiduels) et 20 j après l’injection de PMSG. Le taux de liaison des anticorps anti-PMSG a été estimé par dosage radioimmunologique pour chaque échantillon. Les taux de liaison résiduels augmentent avec l’âge des brebis et ont des effets négatifs sur les performances de reproduction ultérieures : les taux élevés sont associés à de faible fertilité après insémination.
All documents in ORBi are protected by a user license.
[en] Estimation of the long-term consequences on reproduction performance of the oestrus synchronisation treatments that are annually applied to ewes was carried out on nine officially controlled dairy flocks in the Roquefort region of France. A hormonal treatment combining the insertion of a vaginal fluoro-gestone acetate (FGA) sponge for 14 days and the injection of about 500 IU of pregnant mare serum gonadotropin (PMSG) at withdrawal was applied to the ewes in seven of the nine flocks. The ewes in the two other flocks were used as controls. Blood samples were taken from each female just before the treatment (to test for the presence of residual antibodies) and 20 days after the PMSG injection. Anti-PMSG antibody binding rates were calculated for each blood sample. The residual binding rate increased with age and induce negative effects on the following years reproduction performances, ie, they increased the probability that the ewes would not become pregnant.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Bodin, L.; SAGA, INRA, Castanet-Tolosan, France
Drion, Pierre ; Université de Liège - ULiège > Services généraux (Faculté de médecine vétérinaire) > Méth. expér. des anim. de labo et éthique en expér. animale
Remy, Benoit; Université de Liège - ULiège - ULG > Faculté de Médecine Vétérinaire, Physiologie de la Reproduction
Brice, G.; Institut d'Elevage, Castanet-Tolosan, France
Cognié, Yves; Institut Scientifique de Recherche Agronomique - INRA > Station PRMD
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Anti-PMSG in sheep subjected annualy to oestrus synchronisation
Publication date :
1997
Journal title :
Reproduction Nutrition Development
ISSN :
0926-5287
eISSN :
1297-9708
Publisher :
EDP Sciences, Les Ulis, France
Volume :
37
Pages :
651-660
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture
Alwan SF, Boland MP, Goudon I (1988) Detection of antibodies following repeated superovulation in beef heifers. Proc 11th Int Cong on Anim Reprod and Artif Insem 2, 139-141
Armstrong DT, Pfitzner AP, Warnes GM, Seamark R (1983) Superovulation treatments and embryo transfer in Angora goats. J Reprod Fert 67, 403-410
Baril G, Remy B, Vallet JC, Beckers JF (1992a) Observations sur l'utilisation de traitements gonadotropes répétés chez la chèvre laitière. Ann Zootech 41, 291-296
Baril G, Remy B, Vallet JC, Beckers JF (1992b) Effect of repeated use of progestagen-PMSG treatment for estrus control in dairy goats out of breeding season. Reprod Dom Anim 27, 161-168
Bavister BD, Dees C, Schultz R (1986) Refractoriness of Rhesus monkeys to repeated ovarian stimulation by exogenous gonadotropins is caused by non-precipiting antibodies Am J Reprod Immunol Microbiol 11-16
Beckers JF, Remy B, Baril G, Figueiredo IR, Bureau F, Sulon J, Saumande J (1995) Anti-eCG antibodies are transmitted via the colostrum in goats. Theriogenology 43, 165
Brice G (1991) Recherches sur les causes de la baisse de fertilité des brebis agées observée parès synchronisation hormonale des chaleurs et insémination artificielle. Document ITOVIC
Canali C, Boiti C, Zamfiri D, Castellini C, Baltaglini M (1991) ATTIIW Congreso Nazionale ASPA 671-678
Christie WB, Newcomb R, Rowson LEA (1979) Ovulation rate and egg recovery in cattle treated repeatedly with PMSG and prostaglandin. The Vererinary Record 31, 281-283
Clarke IJ (1973) Repeated gonadotrophic stimulation of ewes. NZ Soc Anim Prod 33, 204
Gherardi PG, Martin GB (1978) The effect of multiple injections of PMSG on the ovarian activity of Merino ewes. Proc Aust Soc Anim Prod 12, 160
Hulet CV, Foote WC (1969) Ovulatory response of the ewe to repeated injections of PMSG. J Anim Sci 29, 457-463
Jainudeen HR, Hafez ESE, Golimick PD, Moustafa LF (1966) Antigonadotropins in the serum of cows following repeated therapeutic pregnant mare serum injections. Am J Vet Res 27, 669-675
Maurer RR, Hunt WL, Foote RH (1968) Repeated superovulation following administration of exogenous gonadotropins in Dutch-belted rabbits. J Reprod Fert 15, 93-102
Pignon H, Clegg MT, Cole HH (1960) The formation of antigonadotropin in sheep and its effects on the endocrines and reproductive system. Acta Endocrinol 35, 253-260
Rainio V (1992) Embryo Production in Finssheep, Academic Dissertation. University of Kuopio
Remy B, Baril G, Vallet JC, Dufour R, Chouvet C, Saumande J, Chuplin D, Beckers JF (1991) Are antibodies responsible for a decrease superovulatory response in goats which have been treated repeatedly with porcine follicle-stimulating hormone? Theriogenology 36, 389-399
SAS Institute Inc (1989) SAS/STAT User's Guide, Version 6, Fourth edition, Volume 2, SAS Institute Inc, Cary, NC
Staigmiller RB, Bellows RA, Anderson GB, Seidel G.E Jr, Foote WD, Menino AR Jr, Wright RW Jr (1992) Superovulation of cattle with equine pituitary extract and porcine FSH. Theriogenology 37, 1091-1099
Steelman SL, Pohley FM (1953) Assay of the follicle stimulating hormone based on the augmentation with human chorionic gonadotropin. "Assay on FSH" 53, 604-616
Thorell JI, Johansson BG (1971) Enzymatic iodination of polypeptides with 1251 to high specific activity. Biochem Biophys Acta 251, 363-369
Willet EL, Buckner PJ, McShan WH (1953) Refractorines of cows repeatedly superovulated with gonadotropins. J Dairy Sci 36, 1083-1087
